FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078291950> ?p ?o ?g. }

• W2078291950 endingPage "196" @default.
• W2078291950 startingPage "196" @default.
• W2078291950 abstract "The current inability to culture the entirety of observed bacteria is well known and with the advent of ever more powerful molecular tools, that can survey bacterial communities at previously unattainable depth, the gap in our capacity to culture and define all of these species increases exponentially. This gap has essentially become the rate limiting step in determining how the knowledge of which species are present in a sample can be applied to understand the role of these species in an ecosystem or disease process. A case in point is periodontal disease, which is the most widespread oral disease in dogs. If untreated the disease results in significant pain, eventual loss of the dentition and potentially an increased risk of systemic diseases. Previous molecular based studies have identified the bacterial species associated with periodontal disease in dogs; however without cultured strains from many of these species it has not been possible to study whether they play a role in the disease process. Using a quantitative polymerase chain reaction (qPCR) directed approach a range of microbiological media were screened and optimized to enrich for previously uncultivated target species. A systematic screening methodology was then employed to isolate the species of interest. In cases where the target species were not cultivable in isolation, helper strains grown underneath a nitrocellulose membrane were used to provide the necessary growth factors. This guided media optimization approach enabled the purification of 14 species, 8 of which we had previously been unable to cultivate in isolation. It is also applicable to the targeted isolation of isolates from species that have previously been cultured (for example to study intra-species variation) as demonstrated by the successful isolation of 6 targeted isolates of already cultured species. To our knowledge this is the first time this combination of qPCR guided media optimization, strategic screening and helper strain support has been used successfully to isolate previously uncultured bacteria. This approach can be applied to any uncultured bacterial species where knowledge of their nutritional requirements or low relative abundance impedes their isolation." @default.
• W2078291950 created "2016-06-24" @default.
• W2078291950 creator A5041976491 @default.
• W2078291950 creator A5046452200 @default.
• W2078291950 creator A5058269411 @default.
• W2078291950 creator A5089402429 @default.
• W2078291950 creator A5090938675 @default.
• W2078291950 date "2014-01-01" @default.
• W2078291950 modified "2023-09-23" @default.
• W2078291950 title "The Unculturables: targeted isolation of bacterial species associated with canine periodontal health or disease from dental plaque" @default.
• W2078291950 cites W1533894881 @default.
• W2078291950 cites W1858789088 @default.
• W2078291950 cites W1968420123 @default.
• W2078291950 cites W1994604638 @default.
• W2078291950 cites W2034512485 @default.
• W2078291950 cites W2049556417 @default.
• W2078291950 cites W2057686762 @default.
• W2078291950 cites W2059088776 @default.
• W2078291950 cites W2115831350 @default.
• W2078291950 cites W2128301652 @default.
• W2078291950 cites W2152093511 @default.
• W2078291950 cites W2172297241 @default.
• W2078291950 cites W2302623013 @default.
• W2078291950 doi "https://doi.org/10.1186/1471-2180-14-196" @default.
• W2078291950 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4236526" @default.
• W2078291950 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25085267" @default.
• W2078291950 hasPublicationYear "2014" @default.
• W2078291950 type Work @default.
• W2078291950 sameAs 2078291950 @default.
• W2078291950 citedByCount "14" @default.
• W2078291950 countsByYear W20782919502015 @default.
• W2078291950 countsByYear W20782919502016 @default.
• W2078291950 countsByYear W20782919502017 @default.
• W2078291950 countsByYear W20782919502018 @default.
• W2078291950 countsByYear W20782919502019 @default.
• W2078291950 countsByYear W20782919502020 @default.
• W2078291950 countsByYear W20782919502021 @default.
• W2078291950 countsByYear W20782919502022 @default.
• W2078291950 countsByYear W20782919502023 @default.
• W2078291950 crossrefType "journal-article" @default.
• W2078291950 hasAuthorship W2078291950A5041976491 @default.
• W2078291950 hasAuthorship W2078291950A5046452200 @default.
• W2078291950 hasAuthorship W2078291950A5058269411 @default.
• W2078291950 hasAuthorship W2078291950A5089402429 @default.
• W2078291950 hasAuthorship W2078291950A5090938675 @default.
• W2078291950 hasBestOaLocation W20782919501 @default.
• W2078291950 hasConcept C104317684 @default.
• W2078291950 hasConcept C127413603 @default.
• W2078291950 hasConcept C142724271 @default.
• W2078291950 hasConcept C151730666 @default.
• W2078291950 hasConcept C188126409 @default.
• W2078291950 hasConcept C188198153 @default.
• W2078291950 hasConcept C199343813 @default.
• W2078291950 hasConcept C2775941552 @default.
• W2078291950 hasConcept C2779134260 @default.
• W2078291950 hasConcept C2779914569 @default.
• W2078291950 hasConcept C2780385504 @default.
• W2078291950 hasConcept C2992452445 @default.
• W2078291950 hasConcept C49105822 @default.
• W2078291950 hasConcept C54355233 @default.
• W2078291950 hasConcept C71924100 @default.
• W2078291950 hasConcept C78519656 @default.
• W2078291950 hasConcept C86803240 @default.
• W2078291950 hasConcept C89423630 @default.
• W2078291950 hasConceptScore W2078291950C104317684 @default.
• W2078291950 hasConceptScore W2078291950C127413603 @default.
• W2078291950 hasConceptScore W2078291950C142724271 @default.
• W2078291950 hasConceptScore W2078291950C151730666 @default.
• W2078291950 hasConceptScore W2078291950C188126409 @default.
• W2078291950 hasConceptScore W2078291950C188198153 @default.
• W2078291950 hasConceptScore W2078291950C199343813 @default.
• W2078291950 hasConceptScore W2078291950C2775941552 @default.
• W2078291950 hasConceptScore W2078291950C2779134260 @default.
• W2078291950 hasConceptScore W2078291950C2779914569 @default.
• W2078291950 hasConceptScore W2078291950C2780385504 @default.
• W2078291950 hasConceptScore W2078291950C2992452445 @default.
• W2078291950 hasConceptScore W2078291950C49105822 @default.
• W2078291950 hasConceptScore W2078291950C54355233 @default.
• W2078291950 hasConceptScore W2078291950C71924100 @default.
• W2078291950 hasConceptScore W2078291950C78519656 @default.
• W2078291950 hasConceptScore W2078291950C86803240 @default.
• W2078291950 hasConceptScore W2078291950C89423630 @default.
• W2078291950 hasIssue "1" @default.
• W2078291950 hasLocation W20782919501 @default.
• W2078291950 hasLocation W20782919502 @default.
• W2078291950 hasLocation W20782919503 @default.
• W2078291950 hasLocation W20782919504 @default.
• W2078291950 hasOpenAccess W2078291950 @default.
• W2078291950 hasPrimaryLocation W20782919501 @default.
• W2078291950 hasRelatedWork W1485144325 @default.
• W2078291950 hasRelatedWork W2015176894 @default.
• W2078291950 hasRelatedWork W2093136140 @default.
• W2078291950 hasRelatedWork W2359590405 @default.
• W2078291950 hasRelatedWork W2404896189 @default.
• W2078291950 hasRelatedWork W3013932407 @default.
• W2078291950 hasRelatedWork W3173392772 @default.
• W2078291950 hasRelatedWork W3209444574 @default.
• W2078291950 hasRelatedWork W4290648366 @default.

• next