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- W2078292904 abstract "Vasoactive intestinal polypeptide (VIP) interaction with a 94% pure preparation of monocytes isolated from human peripheral blood was studied by direct binding technique using 3-[125I]tyrosyl-VIP as a tracer ligand. Scatchard analysis of binding data was compatible with two classes of binding sites, one with Kd = 0.25 nM and maximal binding capacity of 16 fmol/106 cells, and another one with Kd = 25 nM and maximal binding capacity of 180 fmol/106 cells. The binding was time-, temperature-, and pH-dependent and was saturable, reversible, and specific. This study has demonstrated that human monocytes have high affinity/low capacity as well as low affinity/high capacity binding sites for VIP. No specific VIP binding was found in pure preparations of human granulocytes, platelets or erythrocytes." @default.
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- W2078292904 date "1985-10-01" @default.
- W2078292904 modified "2023-10-18" @default.
- W2078292904 title "Binding of vasoactive intestinal polypeptide (VIP) by human blood monocytes: demonstration of specific binding sites" @default.
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- W2078292904 doi "https://doi.org/10.1016/0167-0115(85)90195-8" @default.
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