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- W2078302419 abstract "Background: Heamorraghic fevers remain some of the most horrific diseases to impact human beings due to their deadly symptoms, limited treatment options and lack of successful vaccines. Heamorraghic fever agents include Ebola, Marburg, Machupo, Lassa, Junin, Rift Valley Fever, and Crimean Congo Heamorraghic Fever viruses, Yellow Fever virus and the bacterial agent Rickettsia prowazekii. Infection by a Heamorraghic fever agent leads to an acute illness where multiple organs of the body are affected and can result in death. Today outbreaks of the disease are sporadic and occur only within the agent's host species territory. Humans are not the natural reservoir for any of these agents but once infected, we can spread the disease through personal contact. Since we have no vaccines for these agents early detection remains the most important factor in preventing a localized outbreak from spreading internationally. Since outbreaks are unpredictable, a cost effective way of screening human populations for the disease would enable quick identification of new outbreaks. Furthermore, once an outbreak of Heamorraghic fever is identified, correct identification of the responsible agent is essential for treatment. Unfortunately the responsible agent is difficult to distinguish in a clinical setting by symptoms alone. A system for rapid identification of Heamorraghic fevers is required for quick containment and response to these diseases. Methods: At the request of the Department of Homeland Security (DHS), Lawrence Livermore National Laboratory (LLNL) has developed signatures for one-step TaqMan RTPCR detection of seven Heamorraghic fever viral agents [Ebola, Marburg, Machupo, Lassa, Junin, Rift Valley Fever, and Crimean Congo Heamorraghic Fever viruses], Yellow Fever virus and one bacterial agent [Rickettsia prowazekii]. These signatures were designed by finding conserved and specific sequences belonging to each disease agent. They were then tested and validated using Taqman RT-PCR. Results: Testing against near neighbors and over 2500 background samples eliminated potential cross-reactivity and increased specificity of the assay. Signatures that showed no cross reactivity were then tested for their Limit of Detection (LOD) value against their target virus. Conclusion: These signatures will allow the specific and sensitive detection of Heamorraghic fever agents essential for a timely response. Abstracts for SupplementInternational Journal of Infectious DiseasesVol. 14Preview Full-Text PDF Open Archive" @default.
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- W2078302419 date "2010-03-01" @default.
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- W2078302419 title "Navigating Dante's inferno: Creation of signatures for the rapid detection of heamorraghic fever agents" @default.
- W2078302419 doi "https://doi.org/10.1016/j.ijid.2010.02.424" @default.
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