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- W2078338713 abstract "Shu Hua Cheng and Ching Hue! KaoCheng, S. H. and Kao, C. H. 1984. The role of proteolytic enzymes in proteindegradation during senescence of rice leaves. — Physiol. Plant. 62: 231—237.The role of proteolytic enzymes in protein degradation of detached and intact leavesof rice seedlings {Oryza sativa L. cv. Taiching Native 1) during senescence and ofmature leaves during reproductive development was investigated. The amount ofsoluble protein decreased by about 50% in 2,, 4, and 15 days for detached, intact andmature leaves, respectively. Three proteolytic enzyme activities were monitored withpH optima of 4.5 for hemoglobin-digesting proteinase, 5.5 for carboxypeptidase and8.0 for aminopeptidase. No azocoll-digesting proteinase activity could be detected inrice leaves. Dialysis did not alter the activities of any of the three proteolyticenzymes. Acid proteinase activity and aminopeptidase activity were highly unstableduring storage of the enzyme extracts at 4°C. Proteolysis was stimulated by inclusionof meroaptoethanal either in the extraction medium or the assay medium.Acid proteinase, carboxypeptidase and aminopeptidase were all present in detached,intact and mature leaves throughout senescence. There seems to be a direct correla-tion between protein degradation and increases of acid proteinase and carboxypep-tidase activity in seedling leaves (detached and intact) during senescence. In senesc-ing (detached and intact) leaves of seedlings the acid proteinase activity developedfirst, while that of carboxypeptidase developed later. Acid proteinase and carboxy-peptidase may play major roles in protein degradation of leaves from seedlingsduring senescence. During reproduetive development, protein degradation was asso-ciated with decreases in the activities of acid proteinase, carboxypeptidase andaminopeptidase in mature leaves suggesting that the enzymes were less important forprotein degradation in this system. Hence, the role of proteolytic enzymes in proteindegradation during senescence of rice leaves appears to depend largely on the leafsystem used.Additional key -Aminopeptidase, carboxypeptidase, Oryza sativa, proteinase.S. H. Cheng, Botany Dept, Washington Siate Univ., Pullman, WA 99164-^230, USA:C. H. Kao (reprint requests), Dept of Agronomy, National Taiwan Univ., Taipei,Taiwan, Republic of China.IntroductionLeaf senescence is accompanie d by a decrease of pro-tein levels (Thimann 1980). A number of studies havebeen made in recent years to elucidate the control ofthis leaf protein breakdown (Felle r et al. 1977, Martinand Thimann 1972 , Ragster and Chrispeels 1979, 1981,Wittenbach 1978, 1979). Most efforts have been di-rected towards finding out whether the increases inactivity of proteolytic enzymes correlate with decreasesn the protein level during leaf senescence. Some inves--eceived 21 March, 1984; revised 28 May, 1984" @default.
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- W2078338713 date "1984-10-01" @default.
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- W2078338713 title "The role of proteolytic enzymes in protein degradation during senescence of rice leaves" @default.
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- W2078338713 doi "https://doi.org/10.1111/j.1399-3054.1984.tb00376.x" @default.
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