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• W2078338841 abstract "The consumption of green tea is associated with a lower risk of several types of human carcinomas. A number of studies have focused on the possible mechanisms of cancer prevention by tea extracts, especially polyphenols such as epigallocatechin-3-gallate (EGCG).Green tea-derived EGCG was tested in human pancreatic carcinoma cells. The cells (PANC-1, MIA PaCa-2, and BxPC-3) were treated with different doses of EGCG (0, 25, 50, 100, and 200 micromol/L) for 48 hours in culture medium. Proliferation of pancreatic carcinoma cells was measured by means of the WST-1 colorimetric assay. For the study of cell invasion, the cells were incubated with 100 micromol/L EGCG for 2 hours. Then, the cells were added into the cell insert, coated with Matrigel basement membrane matrix. After incubation at 37 degrees C for 24 hours, the cells that had invaded through the Matrigel were counted visually under the microscope.The growth of all three pancreatic carcinoma cells was significantly suppressed by EGCG treatment in a dose-dependent manner. EGCG treatment caused significant suppression of the invasive ability of pancreatic carcinoma cells PANC-1, MIA PaCa-2, and BxPC-3 but did not affect the cell cycle protein cyclin D1.EGCG may be a potent biologic inhibitor of human pancreatic carcinomas, reducing their proliferative and invasive activities." @default.
• W2078338841 created "2016-06-24" @default.
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• W2078338841 date "2002-07-01" @default.
• W2078338841 modified "2023-09-25" @default.
• W2078338841 title "Suppression of Human Pancreatic Carcinoma Cell Growth and Invasion by Epigallocatechin-3-Gallate" @default.
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• W2078338841 doi "https://doi.org/10.1097/00006676-200207000-00012" @default.
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