FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078340394> ?p ?o ?g. }

• W2078340394 endingPage "112" @default.
• W2078340394 startingPage "103" @default.
• W2078340394 abstract "GalAGs (galactosaminoglycans) are one subset of the GAG (glycosaminoglycan) family of chemically heterogeneous polysaccharides that are involved in a wide range of biological processes. These complex biomacromolecules are believed to be responsible for the inhibition of nerve regeneration following injury to the central nervous system. The enzymic degradation of GAG chains in damaged nervous tissue by cABC I (chondroitinase ABC I), a broad-specificity lyase that degrades GalAGs, promotes neural recovery. In the present paper, we report the subcloning of cABC I from Proteus vulgaris, and discuss a simple methodology for the recombinant expression and purification of this enzyme. The originally expressed cABC I clone resulted in an enzyme with negligible activity against a variety of GalAG substrates. Sequencing of the cABC I clone revealed four point mutations at issue with the electron-density data of the cABC I crystal structure. Site-directed mutagenesis produced a clone with restored GalAG-degrading function. We have characterized this enzyme biochemically, including an analysis of its substrate specificity. By coupling structural inspections of cABC I and an evaluation of sequence homology against other GAG-degrading lyases, a set of amino acids was chosen for further study. Mutagenesis studies of these residues resulted in the first experimental evidence of cABC I's active site. This work will facilitate the structure–function characterization of biomedically relevant GalAGs and further the development of therapeutics for nerve regeneration." @default.
• W2078340394 created "2016-06-24" @default.
• W2078340394 creator A5001410017 @default.
• W2078340394 creator A5027287092 @default.
• W2078340394 creator A5030667915 @default.
• W2078340394 creator A5036685879 @default.
• W2078340394 creator A5042657126 @default.
• W2078340394 date "2005-02-08" @default.
• W2078340394 modified "2023-09-30" @default.
• W2078340394 title "Chondroitinase ABC I from <i>Proteus vulgaris</i>: cloning, recombinant expression and active site identification" @default.
• W2078340394 cites W1718462190 @default.
• W2078340394 cites W1965649683 @default.
• W2078340394 cites W1975753413 @default.
• W2078340394 cites W1993119187 @default.
• W2078340394 cites W2000460436 @default.
• W2078340394 cites W2012928251 @default.
• W2078340394 cites W2015167429 @default.
• W2078340394 cites W2015729477 @default.
• W2078340394 cites W2017636720 @default.
• W2078340394 cites W2024091191 @default.
• W2078340394 cites W2048291926 @default.
• W2078340394 cites W2050427350 @default.
• W2078340394 cites W2050744599 @default.
• W2078340394 cites W2059896404 @default.
• W2078340394 cites W2081994248 @default.
• W2078340394 cites W2089382319 @default.
• W2078340394 cites W2090000200 @default.
• W2078340394 cites W2133963518 @default.
• W2078340394 cites W2147645628 @default.
• W2078340394 cites W2150917177 @default.
• W2078340394 cites W2169829977 @default.
• W2078340394 cites W5553889 @default.
• W2078340394 doi "https://doi.org/10.1042/bj20041222" @default.
• W2078340394 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1134771" @default.
• W2078340394 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15691229" @default.
• W2078340394 hasPublicationYear "2005" @default.
• W2078340394 type Work @default.
• W2078340394 sameAs 2078340394 @default.
• W2078340394 citedByCount "86" @default.
• W2078340394 countsByYear W20783403942012 @default.
• W2078340394 countsByYear W20783403942013 @default.
• W2078340394 countsByYear W20783403942014 @default.
• W2078340394 countsByYear W20783403942015 @default.
• W2078340394 countsByYear W20783403942016 @default.
• W2078340394 countsByYear W20783403942017 @default.
• W2078340394 countsByYear W20783403942018 @default.
• W2078340394 countsByYear W20783403942019 @default.
• W2078340394 countsByYear W20783403942020 @default.
• W2078340394 countsByYear W20783403942021 @default.
• W2078340394 countsByYear W20783403942022 @default.
• W2078340394 countsByYear W20783403942023 @default.
• W2078340394 crossrefType "journal-article" @default.
• W2078340394 hasAuthorship W2078340394A5001410017 @default.
• W2078340394 hasAuthorship W2078340394A5027287092 @default.
• W2078340394 hasAuthorship W2078340394A5030667915 @default.
• W2078340394 hasAuthorship W2078340394A5036685879 @default.
• W2078340394 hasAuthorship W2078340394A5042657126 @default.
• W2078340394 hasBestOaLocation W20783403942 @default.
• W2078340394 hasConcept C103408237 @default.
• W2078340394 hasConcept C104317684 @default.
• W2078340394 hasConcept C143065580 @default.
• W2078340394 hasConcept C153911025 @default.
• W2078340394 hasConcept C16318435 @default.
• W2078340394 hasConcept C181199279 @default.
• W2078340394 hasConcept C185592680 @default.
• W2078340394 hasConcept C2780662933 @default.
• W2078340394 hasConcept C40767141 @default.
• W2078340394 hasConcept C41183919 @default.
• W2078340394 hasConcept C547475151 @default.
• W2078340394 hasConcept C55493867 @default.
• W2078340394 hasConcept C86803240 @default.
• W2078340394 hasConceptScore W2078340394C103408237 @default.
• W2078340394 hasConceptScore W2078340394C104317684 @default.
• W2078340394 hasConceptScore W2078340394C143065580 @default.
• W2078340394 hasConceptScore W2078340394C153911025 @default.
• W2078340394 hasConceptScore W2078340394C16318435 @default.
• W2078340394 hasConceptScore W2078340394C181199279 @default.
• W2078340394 hasConceptScore W2078340394C185592680 @default.
• W2078340394 hasConceptScore W2078340394C2780662933 @default.
• W2078340394 hasConceptScore W2078340394C40767141 @default.
• W2078340394 hasConceptScore W2078340394C41183919 @default.
• W2078340394 hasConceptScore W2078340394C547475151 @default.
• W2078340394 hasConceptScore W2078340394C55493867 @default.
• W2078340394 hasConceptScore W2078340394C86803240 @default.
• W2078340394 hasIssue "1" @default.
• W2078340394 hasLocation W20783403941 @default.
• W2078340394 hasLocation W20783403942 @default.
• W2078340394 hasLocation W20783403943 @default.
• W2078340394 hasLocation W20783403944 @default.
• W2078340394 hasOpenAccess W2078340394 @default.
• W2078340394 hasPrimaryLocation W20783403941 @default.
• W2078340394 hasRelatedWork W1964489770 @default.
• W2078340394 hasRelatedWork W1975766203 @default.
• W2078340394 hasRelatedWork W2013630470 @default.
• W2078340394 hasRelatedWork W2017218857 @default.
• W2078340394 hasRelatedWork W2047434710 @default.
• W2078340394 hasRelatedWork W2070681424 @default.
• W2078340394 hasRelatedWork W2124597856 @default.

• next