Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078341502> ?p ?o ?g. }
- W2078341502 endingPage "320" @default.
- W2078341502 startingPage "309" @default.
- W2078341502 abstract "From Didelphis marsupialis serum, two antihemorrhagic proteins were isolated by DEAE-Sephacel, Phenyl-Sepharose and Superdex 200 and characterized. Their masses by mass spectrometry were 40 318 AMU for DM40 and 42 373 and 43 010 AMU for DM43, indicating the presence of isoforms for the last. Molecular masses of 44.8 and 47.3 were obtained by SDS–PAGE, respectively for DM40 and DM43. Both inhibitors showed isoelectric points lower than 3.5 and glycosylation percentages varying from 20.5 to 29.0%, as estimated by chemical deglycosylation and amino acid analysis. N-terminal sequences of the first 17 residues of DM40 and DM43 were identical except for the exchange of R9 for P9. Both were homologous to oprin, a similar inhibitor from Didelphis virginiana serum. No evidence of complex formation between DM40 and DM43 was observed either by native PAGE or gel filtration chromatography. In addition to the antihemorrhagic activity, DM40 and DM43 inhibited the hydrolysis of casein, fibrinogen and fibronectin by Bothrops jararaca venom. DM43 also showed antilethal, antiedematogenic and antihyperalgesic activities. None of the inhibitors showed enzymatic activity on casein. Both proteins formed stable complexes with jararhagin and inhibited its hemorrhagic effect as well as the enzymatic activity of this toxin on fluorogenic substrate." @default.
- W2078341502 created "2016-06-24" @default.
- W2078341502 creator A5010027263 @default.
- W2078341502 creator A5039755087 @default.
- W2078341502 creator A5046521830 @default.
- W2078341502 creator A5051022678 @default.
- W2078341502 creator A5062930446 @default.
- W2078341502 date "2000-05-01" @default.
- W2078341502 modified "2023-10-17" @default.
- W2078341502 title "Isolation and characterization of DM40 and DM43, two snake venom metalloproteinase inhibitors from Didelphis marsupialis serum" @default.
- W2078341502 cites W118185431 @default.
- W2078341502 cites W1488354887 @default.
- W2078341502 cites W1532085934 @default.
- W2078341502 cites W1575068560 @default.
- W2078341502 cites W1625660792 @default.
- W2078341502 cites W1775749144 @default.
- W2078341502 cites W1801249858 @default.
- W2078341502 cites W1966491465 @default.
- W2078341502 cites W1976643373 @default.
- W2078341502 cites W1982098467 @default.
- W2078341502 cites W1983648948 @default.
- W2078341502 cites W1985981054 @default.
- W2078341502 cites W1991063206 @default.
- W2078341502 cites W1992437907 @default.
- W2078341502 cites W1995183948 @default.
- W2078341502 cites W1996165994 @default.
- W2078341502 cites W1996775054 @default.
- W2078341502 cites W1999896738 @default.
- W2078341502 cites W2002338475 @default.
- W2078341502 cites W2004880074 @default.
- W2078341502 cites W2005376830 @default.
- W2078341502 cites W2007165949 @default.
- W2078341502 cites W2007332690 @default.
- W2078341502 cites W2014007136 @default.
- W2078341502 cites W2016789009 @default.
- W2078341502 cites W2017470279 @default.
- W2078341502 cites W2018327701 @default.
- W2078341502 cites W2020921331 @default.
- W2078341502 cites W2022619070 @default.
- W2078341502 cites W2032696432 @default.
- W2078341502 cites W2037895537 @default.
- W2078341502 cites W2038303627 @default.
- W2078341502 cites W2055043387 @default.
- W2078341502 cites W2069095038 @default.
- W2078341502 cites W2082104897 @default.
- W2078341502 cites W2087800480 @default.
- W2078341502 cites W2089880340 @default.
- W2078341502 cites W2090862791 @default.
- W2078341502 cites W2095096518 @default.
- W2078341502 cites W2095583307 @default.
- W2078341502 cites W2100837269 @default.
- W2078341502 cites W2104017463 @default.
- W2078341502 cites W2466770480 @default.
- W2078341502 cites W2614999422 @default.
- W2078341502 cites W28646162 @default.
- W2078341502 doi "https://doi.org/10.1016/s0304-4165(00)00022-2" @default.
- W2078341502 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10779682" @default.
- W2078341502 hasPublicationYear "2000" @default.
- W2078341502 type Work @default.
- W2078341502 sameAs 2078341502 @default.
- W2078341502 citedByCount "55" @default.
- W2078341502 countsByYear W20783415022012 @default.
- W2078341502 countsByYear W20783415022013 @default.
- W2078341502 countsByYear W20783415022014 @default.
- W2078341502 countsByYear W20783415022015 @default.
- W2078341502 countsByYear W20783415022016 @default.
- W2078341502 countsByYear W20783415022017 @default.
- W2078341502 countsByYear W20783415022018 @default.
- W2078341502 countsByYear W20783415022019 @default.
- W2078341502 countsByYear W20783415022020 @default.
- W2078341502 countsByYear W20783415022021 @default.
- W2078341502 countsByYear W20783415022022 @default.
- W2078341502 crossrefType "journal-article" @default.
- W2078341502 hasAuthorship W2078341502A5010027263 @default.
- W2078341502 hasAuthorship W2078341502A5039755087 @default.
- W2078341502 hasAuthorship W2078341502A5046521830 @default.
- W2078341502 hasAuthorship W2078341502A5051022678 @default.
- W2078341502 hasAuthorship W2078341502A5062930446 @default.
- W2078341502 hasConcept C105702510 @default.
- W2078341502 hasConcept C153911025 @default.
- W2078341502 hasConcept C181199279 @default.
- W2078341502 hasConcept C185592680 @default.
- W2078341502 hasConcept C2776725428 @default.
- W2078341502 hasConcept C2777577063 @default.
- W2078341502 hasConcept C2779448229 @default.
- W2078341502 hasConcept C2780837108 @default.
- W2078341502 hasConcept C2909919539 @default.
- W2078341502 hasConcept C43617362 @default.
- W2078341502 hasConcept C50476208 @default.
- W2078341502 hasConcept C55493867 @default.
- W2078341502 hasConcept C58121356 @default.
- W2078341502 hasConcept C86803240 @default.
- W2078341502 hasConcept C96294017 @default.
- W2078341502 hasConceptScore W2078341502C105702510 @default.
- W2078341502 hasConceptScore W2078341502C153911025 @default.
- W2078341502 hasConceptScore W2078341502C181199279 @default.
- W2078341502 hasConceptScore W2078341502C185592680 @default.
- W2078341502 hasConceptScore W2078341502C2776725428 @default.