FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078343471> ?p ?o ?g. }

• W2078343471 endingPage "304" @default.
• W2078343471 startingPage "300" @default.
• W2078343471 abstract "Chlamydia pneumoniae has been identified in arterial atherosclerosis. Aortic valves affected by senile calcific aortic stenosis (SCAS) or calcification of a congenital bicuspid valve (CCBAV) may have interior environments similar to atherosclerosis. This study aimed to detect C. pneumoniae within either SCAS or CCBAV.60 valves showing either SCAS (n=36) or CCABV (n=22) and control valves (n=2) were studied for the presence of C. pneumoniae by the following three techniques: (1) indirect immunofluorescence (IF) was performed on 36 SCAS valves, 22 CCBAV valves and 2 control aortic valves using a HEp-2 cell line infected with C. pneumoniae as a positive control. Negative controls comprised duplicate slides of the same valves with omission of the primary antibody step. A section of human stomach was also used as a negative control. A semiquantitative scoring method was used to grade positive IF staining. (2) Polymerase chain reaction (PCR) was performed on 30 SCAS valves, 20 CCBAV valves and 1 control valve using C. pneumoniae as a positive control and negative controls comprised a Ureaplasma sp. and human DNA from peripheral blood mononuclear cells. (3) Electron microscopy (EM) was performed upon 13 SCAS, 8 CCBAV and 2 control valves.All three methods failed to detect the presence of C. pneumoniae in any of the 60 aortic valves examined. False positive IF staining was encountered in 81% of test valves and in 76% of negative control valve sections (positive in calcified material due to nonspecific binding of FITC-conjugated secondary antibody). No staining was observed in the negative control stomach sections.This study failed to detect C. pneumoniae within aortic valves showing SCAS or CCBAV. Studies using IF alone to detect C. pneumoniae in calcified tissues should be interpreted with caution, since nonspecific binding of FITC-conjugated secondary antibody by calcium in the cusps may be misinterpreted as evidence of Chlamydia. The use of appropriate controls and ancillary methods for the identification of C. pneumoniae are important in this regard." @default.
• W2078343471 created "2016-06-24" @default.
• W2078343471 creator A5084686737 @default.
• W2078343471 date "2002-09-01" @default.
• W2078343471 modified "2023-09-25" @default.
• W2078343471 title "Failure to detect Chlamydia pneumoniae in senile calcific aortic stenosis or calcified congenital bicuspid aortic valve by immunofluorescence, polymerase chain reaction and electron microscopy" @default.
• W2078343471 cites W1634406801 @default.
• W2078343471 cites W1966033005 @default.
• W2078343471 cites W1974672172 @default.
• W2078343471 cites W1997485184 @default.
• W2078343471 cites W2009577833 @default.
• W2078343471 cites W2039182800 @default.
• W2078343471 cites W2046731473 @default.
• W2078343471 cites W2051537874 @default.
• W2078343471 cites W2059384713 @default.
• W2078343471 cites W2078485259 @default.
• W2078343471 doi "https://doi.org/10.1016/s1054-8807(02)00116-3" @default.
• W2078343471 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12361842" @default.
• W2078343471 hasPublicationYear "2002" @default.
• W2078343471 type Work @default.
• W2078343471 sameAs 2078343471 @default.
• W2078343471 citedByCount "20" @default.
• W2078343471 countsByYear W20783434712012 @default.
• W2078343471 countsByYear W20783434712014 @default.
• W2078343471 countsByYear W20783434712019 @default.
• W2078343471 countsByYear W20783434712023 @default.
• W2078343471 crossrefType "journal-article" @default.
• W2078343471 hasAuthorship W2078343471A5084686737 @default.
• W2078343471 hasConcept C142724271 @default.
• W2078343471 hasConcept C164705383 @default.
• W2078343471 hasConcept C203014093 @default.
• W2078343471 hasConcept C2777038808 @default.
• W2078343471 hasConcept C2778419347 @default.
• W2078343471 hasConcept C2780007028 @default.
• W2078343471 hasConcept C2780122209 @default.
• W2078343471 hasConcept C2780309369 @default.
• W2078343471 hasConcept C2780714102 @default.
• W2078343471 hasConcept C2781355080 @default.
• W2078343471 hasConcept C2781414266 @default.
• W2078343471 hasConcept C71924100 @default.
• W2078343471 hasConceptScore W2078343471C142724271 @default.
• W2078343471 hasConceptScore W2078343471C164705383 @default.
• W2078343471 hasConceptScore W2078343471C203014093 @default.
• W2078343471 hasConceptScore W2078343471C2777038808 @default.
• W2078343471 hasConceptScore W2078343471C2778419347 @default.
• W2078343471 hasConceptScore W2078343471C2780007028 @default.
• W2078343471 hasConceptScore W2078343471C2780122209 @default.
• W2078343471 hasConceptScore W2078343471C2780309369 @default.
• W2078343471 hasConceptScore W2078343471C2780714102 @default.
• W2078343471 hasConceptScore W2078343471C2781355080 @default.
• W2078343471 hasConceptScore W2078343471C2781414266 @default.
• W2078343471 hasConceptScore W2078343471C71924100 @default.
• W2078343471 hasIssue "5" @default.
• W2078343471 hasLocation W20783434711 @default.
• W2078343471 hasLocation W20783434712 @default.
• W2078343471 hasOpenAccess W2078343471 @default.
• W2078343471 hasPrimaryLocation W20783434711 @default.
• W2078343471 hasRelatedWork W1965642954 @default.
• W2078343471 hasRelatedWork W2115635888 @default.
• W2078343471 hasRelatedWork W2139512790 @default.
• W2078343471 hasRelatedWork W2153208389 @default.
• W2078343471 hasRelatedWork W2208481010 @default.
• W2078343471 hasRelatedWork W2411895554 @default.
• W2078343471 hasRelatedWork W2593797089 @default.
• W2078343471 hasRelatedWork W3097607593 @default.
• W2078343471 hasRelatedWork W4210497008 @default.
• W2078343471 hasRelatedWork W4380786050 @default.
• W2078343471 hasVolume "11" @default.
• W2078343471 isParatext "false" @default.
• W2078343471 isRetracted "false" @default.
• W2078343471 magId "2078343471" @default.
• W2078343471 workType "article" @default.