Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078343896> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2078343896 endingPage "369" @default.
- W2078343896 startingPage "360" @default.
- W2078343896 abstract "During the development of an HLA-A polymerase chain reaction using sequence-specific oligonucleotide probes (PCR-SSOP) method for the identification of HLA-A*24 and -A*30 alleles, group amplification resulted in the formation of an unusual PCR product in certain individuals. This fragment was approximately 900 bp smaller than the expected product and was also detected in some non-HLA-A*24- and -A*30-positive individuals acting as negative controls for the group specific amplification. Nucleotide sequence analysis of this product identified it as a unique class I gene sequence displaying homology to both primate and human class I A-locus genes. The entire gene was amplified using PCR and the complete DNA sequence information from exon 1 to exon 8, including introns, was determined. A recombination event was identified which results in the fusion of intron 2 with intron 3, causing a deletion of the intervening exon 3 sequence. In addition, there are two cytosine insertions in the poly-cytosine stretch at the start of exon 4 which cause a frameshift and premature termination. The exon 1 and 2 sequences most closely align with the gorilla allele A*0501, displaying only five mismatches. PCR analysis has established that the gene is associated with the following HLA-A types: HLA-A*3001, -A*3301, -A*3303, -A*6802, -A*2901, -A*0203, -A*0205 and -A*31012. Reverse transcription (RT)-PCR analysis of individuals containing this gene failed to detect any mRNA transcription, suggesting that this is a previously undescribed non-expressed class I pseudogene which we have provisionally named HLA-BEL. Its unique gene structure gives a possible insight into the evolutionary pathway that created HLA class I genes." @default.
- W2078343896 created "2016-06-24" @default.
- W2078343896 creator A5020997320 @default.
- W2078343896 creator A5022752899 @default.
- W2078343896 creator A5072462908 @default.
- W2078343896 date "1999-10-01" @default.
- W2078343896 modified "2023-09-25" @default.
- W2078343896 title "Characterisation of a novel HLA-A pseudogene, HLA-BEL, with significant sequence identity with a gorilla MHC class I gene" @default.
- W2078343896 cites W1486111874 @default.
- W2078343896 cites W1493660219 @default.
- W2078343896 cites W1541452673 @default.
- W2078343896 cites W1545494397 @default.
- W2078343896 cites W1566637298 @default.
- W2078343896 cites W1965323808 @default.
- W2078343896 cites W2000070744 @default.
- W2078343896 cites W2013855737 @default.
- W2078343896 cites W2015119986 @default.
- W2078343896 cites W2023554184 @default.
- W2078343896 cites W2037543756 @default.
- W2078343896 cites W2039176902 @default.
- W2078343896 cites W2046788012 @default.
- W2078343896 cites W2048207676 @default.
- W2078343896 cites W2076402077 @default.
- W2078343896 cites W2093134744 @default.
- W2078343896 cites W2102059073 @default.
- W2078343896 cites W2111969548 @default.
- W2078343896 cites W2118094114 @default.
- W2078343896 cites W2121499377 @default.
- W2078343896 cites W2153085701 @default.
- W2078343896 doi "https://doi.org/10.1034/j.1399-0039.1999.540405.x" @default.
- W2078343896 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10551419" @default.
- W2078343896 hasPublicationYear "1999" @default.
- W2078343896 type Work @default.
- W2078343896 sameAs 2078343896 @default.
- W2078343896 citedByCount "12" @default.
- W2078343896 countsByYear W20783438962015 @default.
- W2078343896 countsByYear W20783438962017 @default.
- W2078343896 countsByYear W20783438962019 @default.
- W2078343896 countsByYear W20783438962021 @default.
- W2078343896 countsByYear W20783438962023 @default.
- W2078343896 crossrefType "journal-article" @default.
- W2078343896 hasAuthorship W2078343896A5020997320 @default.
- W2078343896 hasAuthorship W2078343896A5022752899 @default.
- W2078343896 hasAuthorship W2078343896A5072462908 @default.
- W2078343896 hasConcept C104317684 @default.
- W2078343896 hasConcept C141231307 @default.
- W2078343896 hasConcept C147483822 @default.
- W2078343896 hasConcept C153911025 @default.
- W2078343896 hasConcept C188280979 @default.
- W2078343896 hasConcept C34957205 @default.
- W2078343896 hasConcept C36823959 @default.
- W2078343896 hasConcept C49105822 @default.
- W2078343896 hasConcept C54355233 @default.
- W2078343896 hasConcept C61053724 @default.
- W2078343896 hasConcept C84148353 @default.
- W2078343896 hasConcept C84597430 @default.
- W2078343896 hasConcept C86803240 @default.
- W2078343896 hasConcept C94671646 @default.
- W2078343896 hasConceptScore W2078343896C104317684 @default.
- W2078343896 hasConceptScore W2078343896C141231307 @default.
- W2078343896 hasConceptScore W2078343896C147483822 @default.
- W2078343896 hasConceptScore W2078343896C153911025 @default.
- W2078343896 hasConceptScore W2078343896C188280979 @default.
- W2078343896 hasConceptScore W2078343896C34957205 @default.
- W2078343896 hasConceptScore W2078343896C36823959 @default.
- W2078343896 hasConceptScore W2078343896C49105822 @default.
- W2078343896 hasConceptScore W2078343896C54355233 @default.
- W2078343896 hasConceptScore W2078343896C61053724 @default.
- W2078343896 hasConceptScore W2078343896C84148353 @default.
- W2078343896 hasConceptScore W2078343896C84597430 @default.
- W2078343896 hasConceptScore W2078343896C86803240 @default.
- W2078343896 hasConceptScore W2078343896C94671646 @default.
- W2078343896 hasIssue "4" @default.
- W2078343896 hasLocation W20783438961 @default.
- W2078343896 hasLocation W20783438962 @default.
- W2078343896 hasOpenAccess W2078343896 @default.
- W2078343896 hasPrimaryLocation W20783438961 @default.
- W2078343896 hasRelatedWork W1971010738 @default.
- W2078343896 hasRelatedWork W1981393315 @default.
- W2078343896 hasRelatedWork W1999232590 @default.
- W2078343896 hasRelatedWork W2079217427 @default.
- W2078343896 hasRelatedWork W2092554027 @default.
- W2078343896 hasRelatedWork W2131379448 @default.
- W2078343896 hasRelatedWork W2167692816 @default.
- W2078343896 hasRelatedWork W2286567824 @default.
- W2078343896 hasRelatedWork W4233150668 @default.
- W2078343896 hasRelatedWork W4242521457 @default.
- W2078343896 hasVolume "54" @default.
- W2078343896 isParatext "false" @default.
- W2078343896 isRetracted "false" @default.
- W2078343896 magId "2078343896" @default.
- W2078343896 workType "article" @default.