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- W2078349605 abstract "1. The oxidation of [14C16]palmitate and of dl-2-hydroxy[14C16]palmitate by particulate fractions and by acetone-dried powders of young green leaves has been investigated. 2. [14C16]Palmitate was converted to 2-hydroxy[14C]palmitate, [14C]pentadecanoate, 2-hydroxy[14C]pentadecanoate, [14C]myristate and 2-hydroxy[14C]myristate. If 2-hydroxy[12C]palmitate were present in the incubation mixture, the above metabolites were not radioactive. 3. 2-Hydroxy[14C16]palmitate was converted to [14C]pentadecanoate, 2-hydroxy[14C]pentadecanoate and [14C]myristate. If [12C]palmitate were present in the incubation mixture, the above metabolites were still radioactive. 4. The radioactive acids and hydroxy acids were analysed by gas-liquid radiochromatography after methylation and acetylation. 5. The radioactive hydroxy acids were characterised by chemical oxidation to the next lower unsubstituted acid. 6. Imidazole inhibited the α-oxidation of [14C16]palmitate but not of 2-hydroxy[14C16]palmitate. 7. The results establish both the identity of the 2-hydroxy acids and their role as intermediates in the α-oxidation of long-chain fatty acids by leaf systems. They suggest that the reaction sequence in leaves is : RCH2CO2H → RCHOHCO2H → RCHO → RCO2H" @default.
- W2078349605 created "2016-06-24" @default.
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- W2078349605 date "1966-06-01" @default.
- W2078349605 modified "2023-09-25" @default.
- W2078349605 title "The mechanism of α-oxidation in leaves" @default.
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- W2078349605 doi "https://doi.org/10.1016/0005-2760(66)90112-3" @default.
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