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- W2078378100 abstract "The recovery and separation of the integral membrane proteins, the haemagglutinin-neuraminidase (HN) and the fusion protein (F), from a Sendai virus detergent extract were compared on three different ion-exchange high-performance liquid chromatography (IE-HPLC) columns: Mono Q, TSK DEAE-NPR and Zorbax BioSeries SAX. The detergent, either 1-O-n-octyl-beta-glucopyranoside (octylglucoside) or decyl polyethylene glycol-300 (decyl PEG-300), used for extraction of HN and F proteins from the virions, was also present in the elution buffers at a concentration of 0.1%. Recovery of HN and F proteins was primarily dependent on the detergent present in the eluent, resulting in yields of HN varying from 18 to 28 and 56 to 67%, when octylglucoside and decyl PEG-300, respectively, were used. The highest yield for HN protein was obtained by separation on either a Mono Q or a TSK DEAE-NPR column with decyl PEG-300 as the additive. Yields of F protein were lower, and the highest recovery of 46% was found in the presence of decyl PEG-300 by separation on the Mono Q column. Analysis of the fractions by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and by size-exclusion HPLC indicated that the HN protein eluted in the presence of decyl PEG-300 from the Mono Q and the TSK DEAE-NPR columns was obtained in pure form, while the F protein was slightly contaminated with HN. Analysis of the fractions with monoclonal antibodies directed against conformational epitopes of HN and F proteins indicated that after IE-HPLC the conformation of the proteins is largely retained." @default.
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- W2078378100 date "1989-01-01" @default.
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- W2078378100 title "Comparison of ion-exchange high-performance liquid chromatography columns for purification of sendai virus integral membrane proteins" @default.
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- W2078378100 doi "https://doi.org/10.1016/s0021-9673(01)93891-9" @default.
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