FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078378384> ?p ?o ?g. }

• W2078378384 endingPage "155" @default.
• W2078378384 startingPage "147" @default.
• W2078378384 abstract "1 While exposure of smooth muscle cells to sodium nitroprusside (SNP) leads to the development of tolerance to soluble guanylate cyclase (sGC) activation, the mechanisms responsible for this phenomenon in intact cells remain unclear. In the present study, possible mechanisms of tolerance were investigated in a cell culture model where sGC activity was estimated from the accumulation of cyclic GMP in response to 10 μm SNP over a 15 min period in the presence of a phosphodiesterase (PDE) inhibitor. 2 Pretreatment of rat aortic smooth muscle cells with 10–500 μm SNP led to a dose-dependent downregulation of cyclic GMP accumulation upon subsequent SNP stimulation. This effect was evident as early as 2 h following incubation with 10 μm SNP, reached a plateau at 4 h and was blocked by coincubation with 30 μm oxyhaemoglobin. 3 Pretreatment of smooth muscle cells with the PDE inhibitor, zaprinast, resulted in downregulation of the SNP-induced cyclic GMP accumulation in a time- and concentration-dependent manner, that was first evident after 12 h. Moreover, while the zaprinast-induced downregulation of cyclic GMP accumulation was completely inhibited by the protein kinase A (PKA) inhibitor, H89, tolerance to SNP was partially reversed by H89. 4 β1 sGC steady state mRNA levels of S-nitroso N-acetylpenicillamine (SNAP)- or 8Br-cyclic GMP-pretreated cells were unchanged, as indicated by Northern blot analysis. However, Western blot analysis revealed that α1 protein levels were decreased in zaprinast, but not in SNP, SNAP or 8Br-cyclic GMP pretreated cells. 5 While thiol depletion did not prevent the development of tolerance, pretreatment of cells with SNP in the presence of reducing agents partially or completely restored the ability of cells to respond to SNP. 6 We conclude that tolerance to SNP results from two distinct mechanisms: an early onset, NO-mediated event that is reversed by reducing agents and a more delayed, PKA-sensitive process that is mediated through increases in cyclic GMP and a decrease in sGC protein levels." @default.
• W2078378384 created "2016-06-24" @default.
• W2078378384 creator A5056628756 @default.
• W2078378384 creator A5057831537 @default.
• W2078378384 creator A5066062695 @default.
• W2078378384 creator A5077949204 @default.
• W2078378384 date "1996-01-01" @default.
• W2078378384 modified "2023-10-18" @default.
• W2078378384 title "Mechanisms of tolerance to sodium nitroprusside in rat cultured aortic smooth muscle cells" @default.
• W2078378384 cites W1481438484 @default.
• W2078378384 cites W1493953155 @default.
• W2078378384 cites W1512367461 @default.
• W2078378384 cites W1524633237 @default.
• W2078378384 cites W1599529839 @default.
• W2078378384 cites W176913255 @default.
• W2078378384 cites W1830067208 @default.
• W2078378384 cites W1900029203 @default.
• W2078378384 cites W1972751644 @default.
• W2078378384 cites W1979604520 @default.
• W2078378384 cites W1983432658 @default.
• W2078378384 cites W1991347361 @default.
• W2078378384 cites W1992526945 @default.
• W2078378384 cites W1993783817 @default.
• W2078378384 cites W1997074157 @default.
• W2078378384 cites W2014575233 @default.
• W2078378384 cites W2015289160 @default.
• W2078378384 cites W2016764124 @default.
• W2078378384 cites W2019196160 @default.
• W2078378384 cites W2019615010 @default.
• W2078378384 cites W2021271651 @default.
• W2078378384 cites W2025837125 @default.
• W2078378384 cites W2033785962 @default.
• W2078378384 cites W2046160656 @default.
• W2078378384 cites W2048970457 @default.
• W2078378384 cites W2054301087 @default.
• W2078378384 cites W2054836828 @default.
• W2078378384 cites W2063576996 @default.
• W2078378384 cites W2081394289 @default.
• W2078378384 cites W2094301659 @default.
• W2078378384 cites W2095524286 @default.
• W2078378384 cites W2099403486 @default.
• W2078378384 cites W2100157594 @default.
• W2078378384 cites W2105603182 @default.
• W2078378384 cites W2128635872 @default.
• W2078378384 cites W2156588052 @default.
• W2078378384 cites W2175642831 @default.
• W2078378384 cites W2214008998 @default.
• W2078378384 cites W2243132265 @default.
• W2078378384 cites W2244825722 @default.
• W2078378384 cites W2273026710 @default.
• W2078378384 cites W4293247451 @default.
• W2078378384 cites W43833714 @default.
• W2078378384 doi "https://doi.org/10.1111/j.1476-5381.1996.tb15167.x" @default.
• W2078378384 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1909386" @default.
• W2078378384 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8825356" @default.
• W2078378384 hasPublicationYear "1996" @default.
• W2078378384 type Work @default.
• W2078378384 sameAs 2078378384 @default.
• W2078378384 citedByCount "63" @default.
• W2078378384 countsByYear W20783783842013 @default.
• W2078378384 countsByYear W20783783842015 @default.
• W2078378384 countsByYear W20783783842017 @default.
• W2078378384 countsByYear W20783783842018 @default.
• W2078378384 countsByYear W20783783842019 @default.
• W2078378384 countsByYear W20783783842021 @default.
• W2078378384 countsByYear W20783783842023 @default.
• W2078378384 crossrefType "journal-article" @default.
• W2078378384 hasAuthorship W2078378384A5056628756 @default.
• W2078378384 hasAuthorship W2078378384A5057831537 @default.
• W2078378384 hasAuthorship W2078378384A5066062695 @default.
• W2078378384 hasAuthorship W2078378384A5077949204 @default.
• W2078378384 hasBestOaLocation W20783783842 @default.
• W2078378384 hasConcept C104317684 @default.
• W2078378384 hasConcept C121684516 @default.
• W2078378384 hasConcept C126322002 @default.
• W2078378384 hasConcept C127561419 @default.
• W2078378384 hasConcept C134018914 @default.
• W2078378384 hasConcept C173803235 @default.
• W2078378384 hasConcept C181199279 @default.
• W2078378384 hasConcept C185592680 @default.
• W2078378384 hasConcept C2776415932 @default.
• W2078378384 hasConcept C2776766087 @default.
• W2078378384 hasConcept C2776846068 @default.
• W2078378384 hasConcept C2778827303 @default.
• W2078378384 hasConcept C2779610285 @default.
• W2078378384 hasConcept C2779997099 @default.
• W2078378384 hasConcept C41008148 @default.
• W2078378384 hasConcept C519581460 @default.
• W2078378384 hasConcept C55493867 @default.
• W2078378384 hasConcept C62826618 @default.
• W2078378384 hasConcept C71924100 @default.
• W2078378384 hasConcept C86803240 @default.
• W2078378384 hasConceptScore W2078378384C104317684 @default.
• W2078378384 hasConceptScore W2078378384C121684516 @default.
• W2078378384 hasConceptScore W2078378384C126322002 @default.
• W2078378384 hasConceptScore W2078378384C127561419 @default.
• W2078378384 hasConceptScore W2078378384C134018914 @default.
• W2078378384 hasConceptScore W2078378384C173803235 @default.

• next