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• W2078381503 startingPage "4591" @default.
• W2078381503 abstract "The Escherichia coli rpoB gene, which codes for the 1342-residue beta subunit of RNA polymerase (RNAP), contains two dispensable regions centered around codons 300 and 1000. To test whether these regions demarcate domains of the RNAP beta subunit, fragments encoded by segments of rpoB flanking the dispensable regions were individually overexpressed and purified. We show that these beta-subunit polypeptide fragments, when added with purified recombinant beta', sigma, and alpha subunits of RNAP, reconstitute a functional enzyme in vitro. These results demonstrate that the beta subunit is composed of at least three distinct domains and open another avenue for in vitro studies of RNAP assembly and structure." @default.
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• W2078381503 date "1995-05-09" @default.
• W2078381503 modified "2023-09-23" @default.
• W2078381503 title "Assembly of functional Escherichia coli RNA polymerase containing beta subunit fragments." @default.
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• W2078381503 doi "https://doi.org/10.1073/pnas.92.10.4591" @default.
• W2078381503 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/41990" @default.
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