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• W2078388390 abstract "This study aimed to evaluate the clinical utility of applying near-infrared (NIR) Raman spectroscopy and genetic algorithm-partial least squares-discriminant analysis (GA-PLS-DA) to identify biomolecular changes of cervical tissues associated with dysplastic transformation during colposcopic examination. A total of 105 in vivoRaman spectra were measured from 57 cervical sites (35 normal and 22 precancer sites) of 29 patients recruited, in which 65 spectra were from normal sites, while 40 spectra were from cervical precancerous lesions (i.e., 7 low-grade CIN and 33 high-grade CIN). The GA feature selection technique incorporated with PLS was utilized to study the significant biochemical Raman bands for differentiation between normal and precancer cervical tissues. The GA-PLS-DA algorithm with double cross-validation (dCV) identified seven diagnostically significant Raman bands in the ranges of 925–935, 979–999, 1080–1090, 1240–1260, 1320–1340, 1400–1420, and 1625–1645 cm−1 related to proteins, nucleic acids and lipids in tissue, and yielded a diagnostic accuracy of 82.9% (sensitivity of 72.5% (29/40) and specificity of 89.2% (58/65)) for precancer detection. The results of this exploratory study suggest that Raman spectroscopy in conjunction with GA-PLS-DA and dCV methods has the potential to provide clinically significant discrimination between normal and precancer cervical tissues at the molecular level." @default.
• W2078388390 created "2016-06-24" @default.
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• W2078388390 date "2011-01-01" @default.
• W2078388390 modified "2023-10-10" @default.
• W2078388390 title "In vivo diagnosis of cervical precancer using Raman spectroscopy and genetic algorithm techniques" @default.
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• W2078388390 doi "https://doi.org/10.1039/c1an15296c" @default.
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