Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078388631> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2078388631 endingPage "202" @default.
- W2078388631 startingPage "191" @default.
- W2078388631 abstract "Solubilization of structural protein components of red-cell ghosts and alterations of their biological activities by treatment with various solvents was attempted. Ghosts were suspended in the solvents and then separated into supernates and residues by high-speed ultracentrifugation. Treatment with 8 M urea yielded the highest degree of solubilization (60 per cent) and approximately 80 per cent of the protein-bound sialic acid was found in the supernate. Non-urea solvents yielded supernates rich in proteins but low in phosphorus and carbohydrates. Urea starch gel electrophoresis demonstrated the heterogeneity of the solubilized material. Serological studies showed high sensitivity of the Rh0(D)-antigen to treatment with urea, mercaptoethanol and iodoacetamide. The M-antigen was present in all residues and some M activity was found in the urea supernate. No acetylcholin-esterase (AChE) activity was detected in the residue and supernate of stroma treated with 8 M urea. To ascertain the critical molarity, red blood cells and ghosts were incubated at increasing urea concentrations (0.5–6.0 M) and complete loss of AChE activity was observed with 4 M urea. Considerable reduction of AChE (70 per cent) was found after mild reduction and alkylation of stroma. When mercaptans and iodoacetamide were tested separately, no significant effect on AChE activity was observed except with high concentrations of cysteine." @default.
- W2078388631 created "2016-06-24" @default.
- W2078388631 creator A5049196354 @default.
- W2078388631 creator A5073658267 @default.
- W2078388631 date "1968-08-01" @default.
- W2078388631 modified "2023-09-24" @default.
- W2078388631 title "Solubilization and Structural Integrity of the Human Red Cell Membrane" @default.
- W2078388631 cites W1771691146 @default.
- W2078388631 cites W1966396070 @default.
- W2078388631 cites W2002053161 @default.
- W2078388631 cites W2012663069 @default.
- W2078388631 cites W2013481309 @default.
- W2078388631 cites W2021292699 @default.
- W2078388631 cites W2027962342 @default.
- W2078388631 cites W2034370392 @default.
- W2078388631 cites W2037640892 @default.
- W2078388631 cites W2038146864 @default.
- W2078388631 cites W2039247801 @default.
- W2078388631 cites W2039282020 @default.
- W2078388631 cites W2045184782 @default.
- W2078388631 cites W2052664734 @default.
- W2078388631 cites W2055592202 @default.
- W2078388631 cites W2072855558 @default.
- W2078388631 cites W2085864954 @default.
- W2078388631 cites W2119116080 @default.
- W2078388631 cites W2124089608 @default.
- W2078388631 cites W2439732385 @default.
- W2078388631 cites W2513077913 @default.
- W2078388631 cites W261952897 @default.
- W2078388631 cites W4214925781 @default.
- W2078388631 doi "https://doi.org/10.1111/j.1365-2141.1968.tb01529.x" @default.
- W2078388631 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4970903" @default.
- W2078388631 hasPublicationYear "1968" @default.
- W2078388631 type Work @default.
- W2078388631 sameAs 2078388631 @default.
- W2078388631 citedByCount "24" @default.
- W2078388631 crossrefType "journal-article" @default.
- W2078388631 hasAuthorship W2078388631A5049196354 @default.
- W2078388631 hasAuthorship W2078388631A5073658267 @default.
- W2078388631 hasConcept C12554922 @default.
- W2078388631 hasConcept C1491633281 @default.
- W2078388631 hasConcept C185592680 @default.
- W2078388631 hasConcept C2778106830 @default.
- W2078388631 hasConcept C2994365204 @default.
- W2078388631 hasConcept C41625074 @default.
- W2078388631 hasConcept C4863142 @default.
- W2078388631 hasConcept C55493867 @default.
- W2078388631 hasConcept C86803240 @default.
- W2078388631 hasConcept C95444343 @default.
- W2078388631 hasConceptScore W2078388631C12554922 @default.
- W2078388631 hasConceptScore W2078388631C1491633281 @default.
- W2078388631 hasConceptScore W2078388631C185592680 @default.
- W2078388631 hasConceptScore W2078388631C2778106830 @default.
- W2078388631 hasConceptScore W2078388631C2994365204 @default.
- W2078388631 hasConceptScore W2078388631C41625074 @default.
- W2078388631 hasConceptScore W2078388631C4863142 @default.
- W2078388631 hasConceptScore W2078388631C55493867 @default.
- W2078388631 hasConceptScore W2078388631C86803240 @default.
- W2078388631 hasConceptScore W2078388631C95444343 @default.
- W2078388631 hasIssue "2" @default.
- W2078388631 hasLocation W20783886311 @default.
- W2078388631 hasLocation W20783886312 @default.
- W2078388631 hasOpenAccess W2078388631 @default.
- W2078388631 hasPrimaryLocation W20783886311 @default.
- W2078388631 hasRelatedWork W1961048777 @default.
- W2078388631 hasRelatedWork W1964076847 @default.
- W2078388631 hasRelatedWork W2032161274 @default.
- W2078388631 hasRelatedWork W2069222261 @default.
- W2078388631 hasRelatedWork W2077379206 @default.
- W2078388631 hasRelatedWork W2608251028 @default.
- W2078388631 hasRelatedWork W2700688 @default.
- W2078388631 hasRelatedWork W2770102650 @default.
- W2078388631 hasRelatedWork W3110055093 @default.
- W2078388631 hasRelatedWork W2397779121 @default.
- W2078388631 hasVolume "15" @default.
- W2078388631 isParatext "false" @default.
- W2078388631 isRetracted "false" @default.
- W2078388631 magId "2078388631" @default.
- W2078388631 workType "article" @default.