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• W2078395738 startingPage "4263" @default.
• W2078395738 abstract "Thiazole orange was synthetically incorporated into oligonucleotides by using the corresponding phosphoramidite as the building block for automated DNA synthesis. Due to the covalent fixation of the TO dye as a DNA base surrogate, the TO-modified oligonucleotides do not exhibit a significant increase of fluorescence upon hybridization with the counterstrand. However, if 5-nitroindole (NI) is present as a second artificial DNA base (two base pairs away from the TO dye) a fluorescence increase upon DNA hybridization can be observed. That suggests that a short-range photoinduced electron transfer causes the fluorescence quenching in the single strand. The latter result represents a concept that can be transferred to the commercially available Cy3 label. It enables the Cy3 fluorophore to display the DNA hybridization by a fluorescence increase that is normally not observed with this dye." @default.
• W2078395738 created "2016-06-24" @default.
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• W2078395738 date "2008-04-29" @default.
• W2078395738 modified "2023-10-11" @default.
• W2078395738 title "Thiazole Orange and Cy3: Improvement of Fluorescent DNA Probes with Use of Short Range Electron Transfer" @default.
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• W2078395738 doi "https://doi.org/10.1021/jo8004793" @default.
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• W2078395738 hasPublicationYear "2008" @default.
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