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- W2078396860 abstract "Two methods of preparing cell suspension cultures for deep-freezing are demonstrated, i.e. preliminary cultivation with low concentrations of amino acids for Dioscorea cells, and hardening at 2–10°C in the presence of sucrose (7.0–25.0%) for Panax cells. The cells were frozen according to a special program including initiation of crystallization at −5, −7°C with subsequent exposure to similar temperature for 20 min. The frozen cells were successfully recultivated in vitro after storage in liquid nitrogen. Their growth rate and viability do not differ from those of initial cultures. It is shown that the spectrum and amount of steroid compounds, diosgenine in particular, synthesized in Dioscorea suspension cultures do not change after cell storage in liquid nitrogen." @default.
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- W2078396860 date "1984-03-01" @default.
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- W2078396860 title "Recovery of cell cultures and their biosynthetic capacity after storage of Dioscorea deltoidea and Panax ginseng cells in liquid nitrogen" @default.
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- W2078396860 doi "https://doi.org/10.1016/0304-4211(84)90019-1" @default.
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