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- W2078410478 abstract "In the development of enzyme electrodes for NAD-linked dehydrogenase substrates, three alcohol systems were investigated: ethanol, glycol and butan-1-ol. The enzyme electrodes were prepared using the conducting organic salt, NMP TCNQ and a dehydrogenase enzyme. The NADH produced by the enzyme reaction is detected by the organic salt. Electrodes made from the organic salt have been successfully utilised with dehydrogenase enzymes using the NAD/NADH coenzyme couple. These devices can be used to monitor the production or consumption of NADH in different enzyme systems. The ability of the organic conductor to oxidise NADH effectively means that electrodes made from this material can be used to quantify a myriad of biochemical reactions. YEast alcohol dehydrogenase was used for ethanol and butan-1-ol detection and glycerol dehydrogenase from Klebsiella aerogenes FG5 for glycol detection in the electrode system. The influence of membranes on calibration plots for ethanol and butan-1-ol was investigated by testing the electrode without any membrane and with different membranes over it. The sensitivity and reproducibility of measurements of an ethanol membrane electrode were also investigated. To demonstrate the versatility of the conducting organic salt electrode, a calibration plot for glycol was obtained with a different NAD-enzyme system." @default.
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- W2078410478 date "1991-01-01" @default.
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- W2078410478 title "Bioelectrochemical detection of three alcohols based on a conducting organic salt electrode" @default.
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- W2078410478 doi "https://doi.org/10.1016/0956-5663(91)85018-r" @default.
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