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- W2078412453 abstract "Abstract A particulate enzyme preparation from mung bean seedlings ( Phaseolus aureus ) catalysed the incorporation of mannose from GDP- d - 14 C-mannose into the Hemicellulose B fraction. Complete acid hydrolysis of this material liberated all of the radioactivity as mannose. Enzymatic hydrolysis of the 14 C-polymer with a hemicellulase liberated three neutral, diffusable, 14 C-mannose oligosaccharides. One of these, which migrated like a disaccharide, was hydrolysed by β-mannosidase but not by β-glucosidase indicating that mannose was attached by β-linkages. Free glucose was also released by this treatment indicating that the disaccharide was β-mannosyl-glucose. Periodate oxidation of the larger 14 C-oligosaccharides followed by reduction and hydrolysis produced 14 C-erythritol and 14 C-glycerol suggesting that the mannose was attached by 1–4 linkages." @default.
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- W2078412453 date "1971-09-01" @default.
- W2078412453 modified "2023-09-27" @default.
- W2078412453 title "Incorporation of radioactivity from GDP-d-14C-mannose into hemicellulose B" @default.
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- W2078412453 doi "https://doi.org/10.1016/s0031-9422(00)97202-9" @default.
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