Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078425817> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2078425817 endingPage "175" @default.
- W2078425817 startingPage "169" @default.
- W2078425817 abstract "[ <TEX>${beta}$</TEX> ]Xylosidase 효소활성이 높은 균주를 선발하기 위하여 다양한 김치에서 분리된 Leuconostoc 속 젖산균의 <TEX>${beta}$</TEX>-xylosidase 활성을 탐색하였다. 김치에서 분리된 55개의 Leuconostoc 속 젖산균 중 36개의 균주만이 자일로스를 탄소원으로 이용하였으며, 배추김치에서 분리된 Leu. lactis KCTC 13344 균주가 가장 높은 세포내 <TEX>${beta}$</TEX>-xylosidase 효소활성을 나타내었으며, 효소활성은 pH 6, <TEX>$30^{circ}C$</TEX> 반응조건에서 가장 높게 나타났다. <TEX>$Zn^{2+}$</TEX>을 제외한 금속이온은 효소활성을 유의적으로 증가시켰으며, <TEX>$Fe^{2+}$</TEX>은 1 mM의 농도에서 <TEX>${beta}$</TEX>-xylosidase 대조구와 비교하여 효소활성을 약 40% 증가시켰다. 균주를 배양할 때 사용한 탄소원 중 자일로스가 가장 높은 효소활성을 나타내었고, 효소활성을 위한 최적의 자일로스 농도는 30 g/L였다. 단백질 전기영동 및 활성염색을 수행한 결과 분자량이 약 64 kDa인 Leu. lactis KCTC 13344 균주의 <TEX>${beta}$</TEX>-xylosidase는 자일로스에 의하여 발현이 유도되는 것으로 추정되었다. 자일로스가 30 g/L의 농도로 첨가된 MRS 배지에서 Leu. lactis KCTC 13344 균주의 성장은 20시간 후에 최고에 도달하였고, <TEX>${beta}$</TEX>-xylosidase 효소활성은 16시간 후에 최대 <TEX>$7.1{pm}0.3units/mL$</TEX>이었다. 배양 초기에 주입한 자일로스는 약 20% 정도 소모하였고, 젖산과 아세트산은 3.0 g/L 수준으로 생성되었지만 에탄올은 생성되지 않았다. The <TEX>${beta}$</TEX>-xylosidase (EC 3.2.1.37) production capabilities of lactic acid bacteria in the genus Leuconostoc, isolated from a variety of kimchi (fermented vegetables), were examined. The intracellular levels of <TEX>${beta}$</TEX>-xylosidase were similar to the extracellular levels, when most Leuconostoc lactic acid bacteria were grown in a medium containing xylose as the carbon source. Intracellular <TEX>${beta}$</TEX>-xylosidase with a maximum activity of <TEX>$1.2{pm}0.1units/mL$</TEX> (mean<TEX>${pm}$</TEX>standard error) was obtained from Leuconostoc lactis KCTC 13344, which was isolated from fermented Chinese cabbage. The optimum reaction conditions for Leu. lactis KCTC 13344 <TEX>${beta}$</TEX>-xylosidase activity were pH 6.0 and <TEX>$30^{circ}C$</TEX>, and the addition of most divalent cations, except zinc, to the reaction mixture resulted in a slight increase in enzyme activity. Compared with a media containing other carbon sources, the <TEX>${beta}$</TEX>-xylosidase activity was 5 times higher when Leu. lactis KCTC 13344 was grown in a medium containing xylose as carbon source. Zymographic analysis indicated that the synthesis of Leu. lactis KCTC 13344 <TEX>${beta}$</TEX>-xylosidase (approximate size, 64 kDa) is induced by xylose. A maximum intracellular <TEX>${beta}$</TEX>-xylosidase activity of <TEX>$7.1{pm}0.3units/mL$</TEX> was obtained in a batch cultivation in an MRS medium containing 30 g/L xylose." @default.
- W2078425817 created "2016-06-24" @default.
- W2078425817 creator A5025976052 @default.
- W2078425817 creator A5060745216 @default.
- W2078425817 date "2011-04-30" @default.
- W2078425817 modified "2023-10-18" @default.
- W2078425817 title "β-1,4-Xylosidase Activity of Leuconostoc Lactic Acid Bacteria Isolated from Kimchi" @default.
- W2078425817 cites W1524210177 @default.
- W2078425817 cites W1672654663 @default.
- W2078425817 cites W1840797894 @default.
- W2078425817 cites W1940797061 @default.
- W2078425817 cites W1948262403 @default.
- W2078425817 cites W1965822190 @default.
- W2078425817 cites W1967987135 @default.
- W2078425817 cites W1971063695 @default.
- W2078425817 cites W1973109829 @default.
- W2078425817 cites W2009695540 @default.
- W2078425817 cites W2015441163 @default.
- W2078425817 cites W2030144414 @default.
- W2078425817 cites W2034287191 @default.
- W2078425817 cites W2035050825 @default.
- W2078425817 cites W2037044957 @default.
- W2078425817 cites W2044689054 @default.
- W2078425817 cites W2045189668 @default.
- W2078425817 cites W2057282405 @default.
- W2078425817 cites W2076572530 @default.
- W2078425817 cites W2119857760 @default.
- W2078425817 cites W2144634347 @default.
- W2078425817 cites W2154478500 @default.
- W2078425817 cites W2399464238 @default.
- W2078425817 cites W2596393504 @default.
- W2078425817 cites W3188915507 @default.
- W2078425817 cites W854010771 @default.
- W2078425817 doi "https://doi.org/10.9721/kjfst.2011.43.2.169" @default.
- W2078425817 hasPublicationYear "2011" @default.
- W2078425817 type Work @default.
- W2078425817 sameAs 2078425817 @default.
- W2078425817 citedByCount "2" @default.
- W2078425817 countsByYear W20784258172017 @default.
- W2078425817 crossrefType "journal-article" @default.
- W2078425817 hasAuthorship W2078425817A5025976052 @default.
- W2078425817 hasAuthorship W2078425817A5060745216 @default.
- W2078425817 hasBestOaLocation W20784258171 @default.
- W2078425817 hasConcept C100544194 @default.
- W2078425817 hasConcept C185592680 @default.
- W2078425817 hasConcept C2775920511 @default.
- W2078425817 hasConcept C2778859111 @default.
- W2078425817 hasConcept C2780206646 @default.
- W2078425817 hasConcept C2780294150 @default.
- W2078425817 hasConcept C31903555 @default.
- W2078425817 hasConcept C523546767 @default.
- W2078425817 hasConcept C54355233 @default.
- W2078425817 hasConcept C55493867 @default.
- W2078425817 hasConcept C86803240 @default.
- W2078425817 hasConcept C94412978 @default.
- W2078425817 hasConceptScore W2078425817C100544194 @default.
- W2078425817 hasConceptScore W2078425817C185592680 @default.
- W2078425817 hasConceptScore W2078425817C2775920511 @default.
- W2078425817 hasConceptScore W2078425817C2778859111 @default.
- W2078425817 hasConceptScore W2078425817C2780206646 @default.
- W2078425817 hasConceptScore W2078425817C2780294150 @default.
- W2078425817 hasConceptScore W2078425817C31903555 @default.
- W2078425817 hasConceptScore W2078425817C523546767 @default.
- W2078425817 hasConceptScore W2078425817C54355233 @default.
- W2078425817 hasConceptScore W2078425817C55493867 @default.
- W2078425817 hasConceptScore W2078425817C86803240 @default.
- W2078425817 hasConceptScore W2078425817C94412978 @default.
- W2078425817 hasIssue "2" @default.
- W2078425817 hasLocation W20784258171 @default.
- W2078425817 hasOpenAccess W2078425817 @default.
- W2078425817 hasPrimaryLocation W20784258171 @default.
- W2078425817 hasRelatedWork W2114995901 @default.
- W2078425817 hasRelatedWork W2350537558 @default.
- W2078425817 hasRelatedWork W2351597916 @default.
- W2078425817 hasRelatedWork W243094873 @default.
- W2078425817 hasRelatedWork W2788350730 @default.
- W2078425817 hasRelatedWork W2809581837 @default.
- W2078425817 hasRelatedWork W2892286071 @default.
- W2078425817 hasRelatedWork W3176613990 @default.
- W2078425817 hasRelatedWork W3211731240 @default.
- W2078425817 hasRelatedWork W2494676811 @default.
- W2078425817 hasVolume "43" @default.
- W2078425817 isParatext "false" @default.
- W2078425817 isRetracted "false" @default.
- W2078425817 magId "2078425817" @default.
- W2078425817 workType "article" @default.