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- W2078444222 abstract "The Plasmodium parasite possesses two extrachromosomal genomes; the mitochondrial genetic element and the extrachromosomal plastid-like DNA. The latter has only been fully described for one culture strain of P. falciparum. In this study, a rapid procedure for amplifying plastid DNA from dried blood spots of blood infected with different malaria species was developed. PCR amplification of a 595 bp fragment within the plastid-like large subunit ribosomal-RNA (LSU-rRNA) gene was achieved using primers derived from the P. falciparum sequence. The PCR product was observed in all Plasmodium species examined. Sequence analysis of amplified products homologous to an LSU-rRNA fragment of the plastid-like extrachromosomal circle revealed extensive conservation between Plasmodium species including P. falciparum, P. vivax, P. malariae and P. berghei." @default.
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- W2078444222 date "1997-10-01" @default.
- W2078444222 modified "2023-09-27" @default.
- W2078444222 title "Direct PCR amplification and sequence analysis of extrachromosomal Plasmodium DNA from dried blood spots" @default.
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- W2078444222 doi "https://doi.org/10.1016/s0001-706x(97)00080-6" @default.
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