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• W2078457315 abstract "A novel approach to immunoaffinity MS is described wherein antibodies are appended to a patterned gold Biochip surface. The Biochip surface is patterned with an array of concentric immunocapture zones composed of highly hydrophilic central zones surrounded by moderately hydrophilic zones that reside on a non-wetting background, with protein attachment via electrochemically cleavable linkers. After linker cleavage, matrix application forms a discrete spot suitable for MALDI-TOF-MS. Use of the Biochip to purify transthyretin from human serum allowed a distinct resolution of four disulfide conjugates and one truncated form isoforms with good mass resolution and sensitivity." @default.
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• W2078457315 date "2010-10-19" @default.
• W2078457315 modified "2023-10-17" @default.
• W2078457315 title "Biochips that sequentially capture and focus antigens for immunoaffinity MALDI-TOF MS: A new tool for biomarker verification" @default.
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• W2078457315 doi "https://doi.org/10.1002/pmic.201000219" @default.
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