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• W2078466461 abstract "Abstract A rapid, sensitive and selective liquid chromatography/electrospray mass spectrometry (LC/ES‐MS) method was developed for the quantitative determination of the anaesthetic benzodiazepine midazolam (MID) in human saliva and plasma from patients undergoing anesthesia procedures. Biological samples spiked with diazepam‐ d 5 , the internal standard, were extracted into diethyl ether. Compounds were separated on a Xterra® RP 18 column using a mobile phase of acetonitrile/formic acid 0.1% at a flow rate of 0.25 mL/min under a linear gradient. Column effluents were analyzed using MS with an ES source in the positive ionization mode. Calibration curves were linear in the concentration ranges of 1–250 and 0.2–25 ng/mL in plasma and saliva, respectively. The limits of detection were 0.5 ng/mL in plasma and 0.1 ng/mL in saliva, using a 0.5‐mL sample volume. The recoveries of the spiked samples were above 65%. The method was applied to ten real samples from patients undergoing midazolam treatment. Copyright © 2004 John Wiley & Sons, Ltd." @default.
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• W2078466461 date "2004-11-09" @default.
• W2078466461 modified "2023-10-15" @default.
• W2078466461 title "A sensitive, rapid and specific determination of midazolam in human plasma and saliva by liquid chromatography/electrospray mass spectrometry" @default.
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• W2078466461 doi "https://doi.org/10.1002/rcm.1719" @default.
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