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- W2078479986 abstract "A new solid-phase extraction (SPE) matrix with high affinity for the neurotoxin domoic acid (DA) was designed and tested. A computational modelling study led to the selection of 2-(trifluoromethyl)acrylic acid (TFMAA) as a functional monomer capable of imparting affinity towards domoic acid. Polymeric adsorbents containing TFMAA were synthesised and tested in high ionic strength solutions such as urine and seawater. The TFMAA-based polymers demonstrated excellent performance in solid-phase extraction of domoic acid, retaining the toxin while salts and other interfering compounds such as aspartic and glutamic acids were removed by washing and selective elution. It was shown that the TFMAA-based polymer provided the level of purification of domoic acid from urine and seawater acceptable for its quantification by high performance liquid chromatography–mass spectrometry (HPLC–MS) and enzyme-linked immunosorbent assay (ELISA) without any additional pre-concentration and purification steps." @default.
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- W2078479986 date "1990-01-01" @default.
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- W2078479986 title "High-performance liquid chromatographic assay for domoic acid in serum of different species" @default.
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- W2078479986 doi "https://doi.org/10.1016/s0378-4347(00)82538-4" @default.
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