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• W2078480560 abstract "Abstract Background Rhipicephalus sanguineus , known as the brown dog tick, is a common ectoparasite of domestic dogs and can be found worldwide. R. sanguineus is recognized as the primary vector of the etiological agent of canine monocytic ehrlichiosis and canine babesiosis. Here we present the first description of a R. sanguineus salivary gland transcriptome by the production and analysis of 2,034 expressed sequence tags (EST) from two cDNA libraries, one consctructed using mRNA from dissected salivary glands from female ticks fed for 3-5 days (early to mid library, RsSGL1) and the another from ticks fed for 5 days (mid library, RsSGL2), identifying 1,024 clusters of related sequences. Results Based on sequence similarities to nine different databases, we identified transcripts of genes that were further categorized according to function. The category of putative housekeeping genes contained ~56% of the sequences and had on average 2.49 ESTs per cluster, the secreted protein category contained 26.6% of the ESTs and had 2.47 EST's/clusters, while 15.3% of the ESTs, mostly singletons, were not classifiable, and were annotated as unknown function. The secreted category included genes that coded for lipocalins, proteases inhibitors, disintegrins, metalloproteases, immunomodulatory and antiinflammatory proteins, as Evasins and Da-p36, as well as basic-tail and 18.3 kDa proteins, cement proteins, mucins, defensins and antimicrobial peptides. Comparison of the abundance of ESTs from similar contigs of the two salivary gland cDNA libraries allowed the identification of differentially expressed genes, such as genes coding for Evasins and a thrombin inhibitor, which were over expressed in the RsSGL1 (early to mid library) versus RsSGL2 (mid library), indicating their role in inhibition of inflammation at the tick feeding site from the very beginning of the blood meal. Conversely, sequences related to cement (64P), which function has been correlated with tick attachment, was largely expressed in the mid library. Conclusions Our survey provided an insight into the R. sanguineus sialotranscriptome, which can assist the discovery of new targets for anti-tick vaccines, as well as help to identify pharmacologically active proteins." @default.
• W2078480560 created "2016-06-24" @default.
• W2078480560 creator A5016891526 @default.
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• W2078480560 creator A5075415353 @default.
• W2078480560 creator A5088390227 @default.
• W2078480560 creator A5089492746 @default.
• W2078480560 date "2010-07-22" @default.
• W2078480560 modified "2023-10-07" @default.
• W2078480560 title "An insight into the sialotranscriptome of the brown dog tick, Rhipicephalus sanguineus" @default.
• W2078480560 cites W106359879 @default.
• W2078480560 cites W1535820401 @default.
• W2078480560 cites W1540913060 @default.
• W2078480560 cites W1584173134 @default.
• W2078480560 cites W1773083493 @default.
• W2078480560 cites W1962928044 @default.
• W2078480560 cites W1964911435 @default.
• W2078480560 cites W1966484795 @default.
• W2078480560 cites W1971707621 @default.
• W2078480560 cites W1975050432 @default.
• W2078480560 cites W1977099748 @default.
• W2078480560 cites W1978684810 @default.
• W2078480560 cites W1978963918 @default.
• W2078480560 cites W1981436854 @default.
• W2078480560 cites W1981816138 @default.
• W2078480560 cites W1983402347 @default.
• W2078480560 cites W1984225556 @default.
• W2078480560 cites W1987551100 @default.
• W2078480560 cites W1987575401 @default.
• W2078480560 cites W1987622858 @default.
• W2078480560 cites W1991224321 @default.
• W2078480560 cites W1992018680 @default.
• W2078480560 cites W1994834810 @default.
• W2078480560 cites W2001997232 @default.
• W2078480560 cites W2002011866 @default.
• W2078480560 cites W2002367094 @default.
• W2078480560 cites W2003885329 @default.
• W2078480560 cites W2007462084 @default.
• W2078480560 cites W2014468157 @default.
• W2078480560 cites W2014819693 @default.
• W2078480560 cites W2023311663 @default.
• W2078480560 cites W2024428510 @default.
• W2078480560 cites W2024666704 @default.
• W2078480560 cites W2026976118 @default.
• W2078480560 cites W2029275117 @default.
• W2078480560 cites W2029335150 @default.
• W2078480560 cites W2030437369 @default.
• W2078480560 cites W2031406105 @default.
• W2078480560 cites W2031531095 @default.
• W2078480560 cites W2035946377 @default.
• W2078480560 cites W2037169799 @default.
• W2078480560 cites W2037275316 @default.
• W2078480560 cites W2038126024 @default.
• W2078480560 cites W2038438679 @default.
• W2078480560 cites W2039452717 @default.
• W2078480560 cites W2039586544 @default.
• W2078480560 cites W2040292153 @default.
• W2078480560 cites W2040361076 @default.
• W2078480560 cites W2045166945 @default.
• W2078480560 cites W2046011346 @default.
• W2078480560 cites W2047614107 @default.
• W2078480560 cites W2051023574 @default.
• W2078480560 cites W2056444216 @default.
• W2078480560 cites W2059125424 @default.
• W2078480560 cites W2062523228 @default.
• W2078480560 cites W2062577567 @default.
• W2078480560 cites W2062637402 @default.
• W2078480560 cites W2063377311 @default.
• W2078480560 cites W2064044332 @default.
• W2078480560 cites W2065234600 @default.
• W2078480560 cites W2071472192 @default.
• W2078480560 cites W2073849350 @default.
• W2078480560 cites W2073989193 @default.
• W2078480560 cites W2078789592 @default.
• W2078480560 cites W2079877013 @default.
• W2078480560 cites W2080587028 @default.
• W2078480560 cites W2080758220 @default.
• W2078480560 cites W2080792420 @default.
• W2078480560 cites W2082961146 @default.
• W2078480560 cites W2084146420 @default.
• W2078480560 cites W2084278560 @default.
• W2078480560 cites W2086309602 @default.
• W2078480560 cites W2087123990 @default.
• W2078480560 cites W2087269550 @default.
• W2078480560 cites W2089175291 @default.
• W2078480560 cites W2089988218 @default.
• W2078480560 cites W2090062623 @default.
• W2078480560 cites W2095024992 @default.
• W2078480560 cites W2097382368 @default.
• W2078480560 cites W2098920876 @default.
• W2078480560 cites W2121197587 @default.
• W2078480560 cites W2122120273 @default.
• W2078480560 cites W2124876694 @default.
• W2078480560 cites W2134383662 @default.
• W2078480560 cites W2135243832 @default.
• W2078480560 cites W2145171186 @default.

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