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- W2078481969 abstract "A sensitive method (lower detection limit 5 picomoles) is proposed for the determination of sphingoid bases liberated from mammalian glycosphingolipids by acid hydrolysis. The azo dye 4-dimethylaminoazobenzene-4'-sulfonyl chloride reacts with the primary amino group of the sphingosine bases, forming a stable derivative. Excess reagent, which is degraded during the derivatization, and free amino sugars as common hydrolysis products of glycosphingolipids are completely separated by reversed-phase high-performance liquid chromatography. This method was applied to the determination of the glycosphingolipid content of mouse spleen and thymus." @default.
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- W2078481969 date "1983-01-01" @default.
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- W2078481969 title "Sphingosine determination at the picomole level using dimethylaminoazobenzene sulphonyl chloride" @default.
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- W2078481969 doi "https://doi.org/10.1016/s0378-4347(00)86099-5" @default.
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