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• W2078487259 abstract "In this article, the authors provide directions on using the open-source MATLAB applications LSRtrack and LSRanalyze to quantitatively follow the movements of zebrafish larvae in multi-well plates. This article describes a method to quantify the movements of larval zebrafish in multiwell plates, using the open-source MATLAB applications LSRtrack and LSRanalyze. The protocol comprises four stages: generation of high-quality, flatly illuminated video recordings with exposure settings that facilitate object recognition; analysis of the resulting recordings using tools provided in LSRtrack to optimize tracking accuracy and motion detection; analysis of tracking data using LSRanalyze or custom MATLAB scripts; and implementation of validation controls. The method is reliable, automated and flexible, requires <1 h of hands-on work for completion once optimized and shows excellent signal:noise characteristics. The resulting data can be analyzed to determine the following: positional preference; displacement, velocity and acceleration; and duration and frequency of movement events and rest periods. This approach is widely applicable to the analysis of spontaneous or stimulus-evoked zebrafish larval neurobehavioral phenotypes resulting from a broad array of genetic and environmental manipulations, in a multiwell plate format suitable for high-throughput applications." @default.
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• W2078487259 date "2014-06-05" @default.
• W2078487259 modified "2023-09-26" @default.
• W2078487259 title "Quantification of larval zebrafish motor function in multiwell plates using open-source MATLAB applications" @default.
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• W2078487259 doi "https://doi.org/10.1038/nprot.2014.094" @default.
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