FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078496005> ?p ?o ?g. }

• W2078496005 endingPage "7790" @default.
• W2078496005 startingPage "7767" @default.
• W2078496005 abstract "Proline is crucial for energizing critical events throughout the life cycle of Trypanosoma cruzi, the etiological agent of Chagas disease. The proline breakdown pathway consists of two oxidation steps, both of which produce reducing equivalents as follows: the conversion of proline to Δ1-pyrroline-5-carboxylate (P5C), and the subsequent conversion of P5C to glutamate. We have identified and characterized the Δ1-pyrroline-5-carboxylate dehydrogenase from T. cruzi (TcP5CDH) and report here on how this enzyme contributes to a central metabolic pathway in this parasite. Size-exclusion chromatography, two-dimensional gel electrophoresis, and small angle x-ray scattering analysis of TcP5CDH revealed an oligomeric state composed of two subunits of six protomers. TcP5CDH was found to complement a yeast strain deficient in PUT2 activity, confirming the enzyme's functional role; and the biochemical parameters (Km, kcat, and kcat/Km) of the recombinant TcP5CDH were determined, exhibiting values comparable with those from T. cruzi lysates. In addition, TcP5CDH exhibited mitochondrial staining during the main stages of the T. cruzi life cycle. mRNA and enzymatic activity levels indicated the up-regulation (6-fold change) of TcP5CDH during the infective stages of the parasite. The participation of P5C as an energy source was also demonstrated. Overall, we propose that this enzymatic step is crucial for the viability of both replicative and infective forms of T. cruzi. Proline is crucial for energizing critical events throughout the life cycle of Trypanosoma cruzi, the etiological agent of Chagas disease. The proline breakdown pathway consists of two oxidation steps, both of which produce reducing equivalents as follows: the conversion of proline to Δ1-pyrroline-5-carboxylate (P5C), and the subsequent conversion of P5C to glutamate. We have identified and characterized the Δ1-pyrroline-5-carboxylate dehydrogenase from T. cruzi (TcP5CDH) and report here on how this enzyme contributes to a central metabolic pathway in this parasite. Size-exclusion chromatography, two-dimensional gel electrophoresis, and small angle x-ray scattering analysis of TcP5CDH revealed an oligomeric state composed of two subunits of six protomers. TcP5CDH was found to complement a yeast strain deficient in PUT2 activity, confirming the enzyme's functional role; and the biochemical parameters (Km, kcat, and kcat/Km) of the recombinant TcP5CDH were determined, exhibiting values comparable with those from T. cruzi lysates. In addition, TcP5CDH exhibited mitochondrial staining during the main stages of the T. cruzi life cycle. mRNA and enzymatic activity levels indicated the up-regulation (6-fold change) of TcP5CDH during the infective stages of the parasite. The participation of P5C as an energy source was also demonstrated. Overall, we propose that this enzymatic step is crucial for the viability of both replicative and infective forms of T. cruzi." @default.
• W2078496005 created "2016-06-24" @default.
• W2078496005 creator A5031932976 @default.
• W2078496005 creator A5034138074 @default.
• W2078496005 creator A5041380144 @default.
• W2078496005 creator A5043211179 @default.
• W2078496005 creator A5059638044 @default.
• W2078496005 creator A5063787649 @default.
• W2078496005 creator A5079106730 @default.
• W2078496005 creator A5082911188 @default.
• W2078496005 date "2015-03-01" @default.
• W2078496005 modified "2023-10-16" @default.
• W2078496005 title "Role of Δ1-Pyrroline-5-Carboxylate Dehydrogenase Supports Mitochondrial Metabolism and Host-Cell Invasion of Trypanosoma cruzi" @default.
• W2078496005 cites W1529976568 @default.
• W2078496005 cites W1556017032 @default.
• W2078496005 cites W1584638963 @default.
• W2078496005 cites W1587373255 @default.
• W2078496005 cites W1681177208 @default.
• W2078496005 cites W1709246161 @default.
• W2078496005 cites W1764031643 @default.
• W2078496005 cites W1840703237 @default.
• W2078496005 cites W1964219100 @default.
• W2078496005 cites W1966753532 @default.
• W2078496005 cites W1975309292 @default.
• W2078496005 cites W1976776701 @default.
• W2078496005 cites W1977038370 @default.
• W2078496005 cites W1978874279 @default.
• W2078496005 cites W1979986507 @default.
• W2078496005 cites W1981169853 @default.
• W2078496005 cites W1987202538 @default.
• W2078496005 cites W1988932135 @default.
• W2078496005 cites W1989796495 @default.
• W2078496005 cites W1991357892 @default.
• W2078496005 cites W1991691234 @default.
• W2078496005 cites W1997291201 @default.
• W2078496005 cites W1999133653 @default.
• W2078496005 cites W1999671103 @default.
• W2078496005 cites W1999872755 @default.
• W2078496005 cites W2002033007 @default.
• W2078496005 cites W2004397421 @default.
• W2078496005 cites W2008162817 @default.
• W2078496005 cites W2010032118 @default.
• W2078496005 cites W2015617558 @default.
• W2078496005 cites W2018062352 @default.
• W2078496005 cites W2019945640 @default.
• W2078496005 cites W2022492040 @default.
• W2078496005 cites W2025142599 @default.
• W2078496005 cites W2032349840 @default.
• W2078496005 cites W2033799632 @default.
• W2078496005 cites W2036869932 @default.
• W2078496005 cites W2038410430 @default.
• W2078496005 cites W2038591876 @default.
• W2078496005 cites W2042505132 @default.
• W2078496005 cites W2044179749 @default.
• W2078496005 cites W2044212453 @default.
• W2078496005 cites W2044904826 @default.
• W2078496005 cites W2045468030 @default.
• W2078496005 cites W2045594331 @default.
• W2078496005 cites W2046893571 @default.
• W2078496005 cites W2049102964 @default.
• W2078496005 cites W2051482261 @default.
• W2078496005 cites W2052113480 @default.
• W2078496005 cites W2064563279 @default.
• W2078496005 cites W2065752585 @default.
• W2078496005 cites W2066088601 @default.
• W2078496005 cites W2066371247 @default.
• W2078496005 cites W2067900128 @default.
• W2078496005 cites W2068584243 @default.
• W2078496005 cites W2072356380 @default.
• W2078496005 cites W2072435127 @default.
• W2078496005 cites W2074813964 @default.
• W2078496005 cites W2076279650 @default.
• W2078496005 cites W2078704305 @default.
• W2078496005 cites W2079380454 @default.
• W2078496005 cites W2080408951 @default.
• W2078496005 cites W2082071395 @default.
• W2078496005 cites W2082086399 @default.
• W2078496005 cites W2085025870 @default.
• W2078496005 cites W2085101416 @default.
• W2078496005 cites W2089526897 @default.
• W2078496005 cites W2090880001 @default.
• W2078496005 cites W2091413967 @default.
• W2078496005 cites W2091664813 @default.
• W2078496005 cites W2094019524 @default.
• W2078496005 cites W2100717661 @default.
• W2078496005 cites W2100837269 @default.
• W2078496005 cites W2102388801 @default.
• W2078496005 cites W2104023491 @default.
• W2078496005 cites W2106882534 @default.
• W2078496005 cites W2115073295 @default.
• W2078496005 cites W2116354728 @default.
• W2078496005 cites W2116540187 @default.
• W2078496005 cites W2119845886 @default.
• W2078496005 cites W2122547807 @default.
• W2078496005 cites W2137226072 @default.
• W2078496005 cites W2139993395 @default.
• W2078496005 cites W2140333559 @default.
• W2078496005 cites W2143789570 @default.

• next