Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078504811> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W2078504811 abstract "Accurate diagnostic tests are powerful tool to control the spread of the human T-lymphotropic virus (HTLV) infection. In Brazil, the currently applied diagnostic algorithm for HTLV-1/2 screening is based on serological tests (enzyme-linked immunosorbent assay followed by Western Blot). However this algorithm is unsuitable due to its high cost and the elevated rate of Western Blot (WB) indeterminate results. Nevertheless, molecular techniques such as real-time PCR (qPCR) can overlap these drawbacks because of their high sensitivity and specificity. Several studies have described different qPCR protocols for HTLV-1/2 diagnosis but they lack suitable validation. For this reason, we developed and validated a qualitative multiplex qPCR platform for simultaneous detection and discrimination of the HTLV-1/2 infection in a single reaction tube. A total of 73 HTLV-positive samples and 100 samples obtained from non-infected individuals were tested. The detection limit was one copy/reaction for HTLV-1 and 10 copies/reaction for HTLV-2. We observed a high and similar efficiency between the single and multiplex format as well as the cycle treshold and r2 values. In addition, this molecular platform reached 100% of sensitivity and specificity. In conclusion, the developed method using one tube multiplex qPCR (confirmatory and discriminatory) for HTLV-1/2 was validated. It showed low cost and high sensitivity and specificity compared to previously described assays and to the traditional confirmatory method (WB). Therefore, this platform can be a supportive tool for the current confirmatory methods adopted." @default.
- W2078504811 created "2016-06-24" @default.
- W2078504811 creator A5014894298 @default.
- W2078504811 creator A5019391041 @default.
- W2078504811 creator A5021887908 @default.
- W2078504811 creator A5052312845 @default.
- W2078504811 creator A5056507028 @default.
- W2078504811 creator A5064146570 @default.
- W2078504811 creator A5064437978 @default.
- W2078504811 creator A5076026195 @default.
- W2078504811 creator A5083772823 @default.
- W2078504811 date "2014-01-01" @default.
- W2078504811 modified "2023-09-26" @default.
- W2078504811 title "Development and validation of a Multiplex Real-Time PCR for HTLV-1/2 confirmatory diagnosis" @default.
- W2078504811 doi "https://doi.org/10.1186/1742-4690-11-s1-p105" @default.
- W2078504811 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4043228" @default.
- W2078504811 hasPublicationYear "2014" @default.
- W2078504811 type Work @default.
- W2078504811 sameAs 2078504811 @default.
- W2078504811 citedByCount "1" @default.
- W2078504811 countsByYear W20785048112016 @default.
- W2078504811 crossrefType "journal-article" @default.
- W2078504811 hasAuthorship W2078504811A5014894298 @default.
- W2078504811 hasAuthorship W2078504811A5019391041 @default.
- W2078504811 hasAuthorship W2078504811A5021887908 @default.
- W2078504811 hasAuthorship W2078504811A5052312845 @default.
- W2078504811 hasAuthorship W2078504811A5056507028 @default.
- W2078504811 hasAuthorship W2078504811A5064146570 @default.
- W2078504811 hasAuthorship W2078504811A5064437978 @default.
- W2078504811 hasAuthorship W2078504811A5076026195 @default.
- W2078504811 hasAuthorship W2078504811A5083772823 @default.
- W2078504811 hasBestOaLocation W20785048111 @default.
- W2078504811 hasConcept C119857082 @default.
- W2078504811 hasConcept C159047783 @default.
- W2078504811 hasConcept C159654299 @default.
- W2078504811 hasConcept C203014093 @default.
- W2078504811 hasConcept C2781188995 @default.
- W2078504811 hasConcept C40722632 @default.
- W2078504811 hasConcept C41008148 @default.
- W2078504811 hasConcept C60644358 @default.
- W2078504811 hasConcept C70721500 @default.
- W2078504811 hasConcept C71104824 @default.
- W2078504811 hasConcept C71924100 @default.
- W2078504811 hasConcept C86803240 @default.
- W2078504811 hasConceptScore W2078504811C119857082 @default.
- W2078504811 hasConceptScore W2078504811C159047783 @default.
- W2078504811 hasConceptScore W2078504811C159654299 @default.
- W2078504811 hasConceptScore W2078504811C203014093 @default.
- W2078504811 hasConceptScore W2078504811C2781188995 @default.
- W2078504811 hasConceptScore W2078504811C40722632 @default.
- W2078504811 hasConceptScore W2078504811C41008148 @default.
- W2078504811 hasConceptScore W2078504811C60644358 @default.
- W2078504811 hasConceptScore W2078504811C70721500 @default.
- W2078504811 hasConceptScore W2078504811C71104824 @default.
- W2078504811 hasConceptScore W2078504811C71924100 @default.
- W2078504811 hasConceptScore W2078504811C86803240 @default.
- W2078504811 hasIssue "S1" @default.
- W2078504811 hasLocation W20785048111 @default.
- W2078504811 hasLocation W20785048112 @default.
- W2078504811 hasOpenAccess W2078504811 @default.
- W2078504811 hasPrimaryLocation W20785048111 @default.
- W2078504811 hasRelatedWork W2024080387 @default.
- W2078504811 hasRelatedWork W2028779135 @default.
- W2078504811 hasRelatedWork W2067397386 @default.
- W2078504811 hasRelatedWork W2083885137 @default.
- W2078504811 hasRelatedWork W2109521977 @default.
- W2078504811 hasRelatedWork W2163895276 @default.
- W2078504811 hasRelatedWork W2531059238 @default.
- W2078504811 hasRelatedWork W2912110029 @default.
- W2078504811 hasRelatedWork W3032451335 @default.
- W2078504811 hasRelatedWork W4211080546 @default.
- W2078504811 hasVolume "11" @default.
- W2078504811 isParatext "false" @default.
- W2078504811 isRetracted "false" @default.
- W2078504811 magId "2078504811" @default.
- W2078504811 workType "article" @default.