Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078512342> ?p ?o ?g. }
- W2078512342 abstract "Kinesin-1 is a dimeric motor that transports cargo along microtubules, taking 8.2-nm steps in a hand-over-hand fashion. The ATP hydrolysis cycles of its two heads are maintained out of phase by a series of gating mechanisms, which lead to processive runs averaging ∼1 μm. A key structural element for inter-head coordination is the neck linker (NL), which connects the heads to the stalk. To examine the role of the NL in regulating stepping, we investigated NL mutants of various lengths using single-molecule optical trapping and bulk fluorescence approaches in the context of a general framework for gating. Our results show that, although inter-head tension enhances motor velocity, it is crucial neither for inter-head coordination nor for rapid rear-head release. Furthermore, cysteine-light mutants do not produce wild-type motility under load. We conclude that kinesin-1 is primarily front-head gated, and that NL length is tuned to enhance unidirectional processivity and velocity." @default.
- W2078512342 created "2016-06-24" @default.
- W2078512342 creator A5001285523 @default.
- W2078512342 creator A5007662018 @default.
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- W2078512342 creator A5030952221 @default.
- W2078512342 creator A5047173144 @default.
- W2078512342 creator A5085429881 @default.
- W2078512342 date "2015-04-22" @default.
- W2078512342 modified "2023-10-01" @default.
- W2078512342 title "Examining kinesin processivity within a general gating framework" @default.
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- W2078512342 doi "https://doi.org/10.7554/elife.07403" @default.
- W2078512342 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4453223" @default.
- W2078512342 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25902401" @default.
- W2078512342 hasPublicationYear "2015" @default.
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