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- W2078542512 abstract "We have cloned a new insulin-like growth factors binding protein (IGFBP) from a human osteosarcoma cDNA library. Two conserved regions in the COOH-terminal third of the five known human IGFBPs were used to design primers and to perform polymerase chain reaction (PCR) with osteosarcoma cDNA as a template. One of the eight PCR products encoded a unique IGFBP sequence. The DNA sequence was used to synthesize probes to screen an osteosarcoma cDNA library and isolate full length cDNA clones. The amino acid sequence was deduced from one of them. It contains two possible signal peptidase cleavage sites yielding a mature molecule of 257 or 252 amino acids, and 18 cysteines in identical positions to the other IGFBPs. The most pronounced homology exists with human IGFBP-3 (50 % in the NH2− and 45 % in the COOH-terminal region)." @default.
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- W2078542512 date "1991-04-01" @default.
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- W2078542512 title "Molecular cloning of a new human insulin-like growth factor binding protein" @default.
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- W2078542512 doi "https://doi.org/10.1016/0006-291x(91)90912-q" @default.
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