Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078550641> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2078550641 endingPage "258" @default.
- W2078550641 startingPage "252" @default.
- W2078550641 abstract "Poly(ADP-ribose) built from NAD+ on histones and other nuclear proteins by poly(ADP-ribose) polymerase is involved in repair, replication, gene expression, recombination, and chromatin remodeling in embryogenesis. Such nuclear processes are believed to be facilitated by opening up of condensed chromatin structures and by removal of histones from DNA at damaged sites as well as at origins of replication and transcription initiation sites. In addition, poly(ADP-ribosyl)ation might be involved in the up or down regulation of the activity of key nuclear enzymes. Poly(ADP-ribose) is rapidly synthesized at sites containing DNA strand breaks and is then rapidly degraded (half-life 0.5-5 min) by poly(ADP-ribose)glycohydrolase. High-resolution polyacrylamide gel electrophoresis is used in this study to analyze the rate of consumption of [32P]NAD+, the rate of formation of poly(ADP-ribose) molecules, and the rate of appearance of ADP-ribose, AMP, and phosphoribosyl-AMP, the catabolites of poly(ADP-ribose) in isolated nuclei from mouse cells in culture. Our method permits direct loading of aliquots of nuclei at time intervals on the polyacrylamide gel. The action of poly(ADP-ribose) glycohydrolase that degrades the polymer starts at less than 2 min from polymer formation. A poly(ADP-ribose) phosphodiesterase present in mammalian cell nuclei begins degrading poly(ADP-ribose) or unincorporated NAD+ and free ADP-ribose at 10 min. Mammalian phosphodiesterase is identified as an enzyme more important than previously thought which might degrade poly(ADP-ribosyl)ated proteins but also recycle the ADP-ribose produced from di- to poly(ADP-ribosyl)ated proteins by glycohydrolase into utilizable AMP units." @default.
- W2078550641 created "2016-06-24" @default.
- W2078550641 creator A5003061150 @default.
- W2078550641 date "1992-06-01" @default.
- W2078550641 modified "2023-10-16" @default.
- W2078550641 title "Poly(ADP-ribose) synthesis and degradation in mammalian nuclei" @default.
- W2078550641 cites W1479921968 @default.
- W2078550641 cites W1481011810 @default.
- W2078550641 cites W1490482172 @default.
- W2078550641 cites W1494736908 @default.
- W2078550641 cites W1498308778 @default.
- W2078550641 cites W1515916437 @default.
- W2078550641 cites W1536009441 @default.
- W2078550641 cites W1546212643 @default.
- W2078550641 cites W1552451187 @default.
- W2078550641 cites W1608617260 @default.
- W2078550641 cites W1662942466 @default.
- W2078550641 cites W171635663 @default.
- W2078550641 cites W1964145596 @default.
- W2078550641 cites W1967439169 @default.
- W2078550641 cites W1992975619 @default.
- W2078550641 cites W1998843941 @default.
- W2078550641 cites W2003345927 @default.
- W2078550641 cites W2005845638 @default.
- W2078550641 cites W2069640985 @default.
- W2078550641 cites W2077559325 @default.
- W2078550641 cites W2093583140 @default.
- W2078550641 cites W2164345529 @default.
- W2078550641 cites W2345134020 @default.
- W2078550641 cites W4252215306 @default.
- W2078550641 cites W96993440 @default.
- W2078550641 doi "https://doi.org/10.1016/0003-2697(92)90310-4" @default.
- W2078550641 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1329575" @default.
- W2078550641 hasPublicationYear "1992" @default.
- W2078550641 type Work @default.
- W2078550641 sameAs 2078550641 @default.
- W2078550641 citedByCount "17" @default.
- W2078550641 countsByYear W20785506412012 @default.
- W2078550641 countsByYear W20785506412013 @default.
- W2078550641 countsByYear W20785506412019 @default.
- W2078550641 crossrefType "journal-article" @default.
- W2078550641 hasAuthorship W2078550641A5003061150 @default.
- W2078550641 hasConcept C138885662 @default.
- W2078550641 hasConcept C153911025 @default.
- W2078550641 hasConcept C179926584 @default.
- W2078550641 hasConcept C181199279 @default.
- W2078550641 hasConcept C182979987 @default.
- W2078550641 hasConcept C185592680 @default.
- W2078550641 hasConcept C2779461342 @default.
- W2078550641 hasConcept C41895202 @default.
- W2078550641 hasConcept C45510557 @default.
- W2078550641 hasConcept C552990157 @default.
- W2078550641 hasConcept C55493867 @default.
- W2078550641 hasConcept C64927066 @default.
- W2078550641 hasConcept C75520062 @default.
- W2078550641 hasConcept C82381507 @default.
- W2078550641 hasConcept C83640560 @default.
- W2078550641 hasConcept C86803240 @default.
- W2078550641 hasConceptScore W2078550641C138885662 @default.
- W2078550641 hasConceptScore W2078550641C153911025 @default.
- W2078550641 hasConceptScore W2078550641C179926584 @default.
- W2078550641 hasConceptScore W2078550641C181199279 @default.
- W2078550641 hasConceptScore W2078550641C182979987 @default.
- W2078550641 hasConceptScore W2078550641C185592680 @default.
- W2078550641 hasConceptScore W2078550641C2779461342 @default.
- W2078550641 hasConceptScore W2078550641C41895202 @default.
- W2078550641 hasConceptScore W2078550641C45510557 @default.
- W2078550641 hasConceptScore W2078550641C552990157 @default.
- W2078550641 hasConceptScore W2078550641C55493867 @default.
- W2078550641 hasConceptScore W2078550641C64927066 @default.
- W2078550641 hasConceptScore W2078550641C75520062 @default.
- W2078550641 hasConceptScore W2078550641C82381507 @default.
- W2078550641 hasConceptScore W2078550641C83640560 @default.
- W2078550641 hasConceptScore W2078550641C86803240 @default.
- W2078550641 hasIssue "2" @default.
- W2078550641 hasLocation W20785506411 @default.
- W2078550641 hasLocation W20785506412 @default.
- W2078550641 hasOpenAccess W2078550641 @default.
- W2078550641 hasPrimaryLocation W20785506411 @default.
- W2078550641 hasRelatedWork W1964264712 @default.
- W2078550641 hasRelatedWork W1968609213 @default.
- W2078550641 hasRelatedWork W2006531948 @default.
- W2078550641 hasRelatedWork W2016517525 @default.
- W2078550641 hasRelatedWork W2051063130 @default.
- W2078550641 hasRelatedWork W2108899241 @default.
- W2078550641 hasRelatedWork W2887912390 @default.
- W2078550641 hasRelatedWork W4234754696 @default.
- W2078550641 hasRelatedWork W4252517065 @default.
- W2078550641 hasRelatedWork W4362522364 @default.
- W2078550641 hasVolume "203" @default.
- W2078550641 isParatext "false" @default.
- W2078550641 isRetracted "false" @default.
- W2078550641 magId "2078550641" @default.
- W2078550641 workType "article" @default.