Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078558208> ?p ?o ?g. }
- W2078558208 endingPage "405" @default.
- W2078558208 startingPage "391" @default.
- W2078558208 abstract "Recent human clinical trials results demonstrated successful treatment for certain genetic forms of cystic fibrosis (CF). To extend treatment opportunities to those afflicted with other genetic forms of CF disease, structural and biophysical characterization of CF transmembrane conductance regulator (CFTR) is urgently needed. In this study, CFTR was modified with various tags, including a His10 purification tag, the SUMOstar (SUMO*) domain, an extracellular FLAG epitope, and an enhanced green fluorescent protein (EGFP), each alone or in various combinations. Expressed in HEK293 cells, recombinant CFTR proteins underwent complex glycosylation, compartmentalized with the plasma membrane, and exhibited regulated chloride-channel activity with only modest alterations in channel conductance and gating kinetics. Surface CFTR expression level was enhanced by the presence of SUMO* on the N-terminus. Quantitative mass-spectrometric analysis indicated approximately 10 % of the total recombinant CFTR (SUMO*–CFTRFLAG–EGFP) localized to the plasma membrane. Trial purification using dodecylmaltoside for membrane protein extraction reproducibly recovered 178 ± 56 μg SUMO*–CFTRFLAG–EGFP per billion cells at 80 % purity. Fluorescence size-exclusion chromatography indicated purified CFTR was monodisperse. These findings demonstrate a stable mammalian cell expression system capable of producing human CFTR of sufficient quality and quantity to augment future CF drug discovery efforts, including biophysical and structural studies." @default.
- W2078558208 created "2016-06-24" @default.
- W2078558208 creator A5022159124 @default.
- W2078558208 creator A5023676292 @default.
- W2078558208 creator A5026711433 @default.
- W2078558208 creator A5031911309 @default.
- W2078558208 creator A5033378804 @default.
- W2078558208 creator A5037376623 @default.
- W2078558208 creator A5045703187 @default.
- W2078558208 creator A5049400835 @default.
- W2078558208 creator A5049704229 @default.
- W2078558208 creator A5053247069 @default.
- W2078558208 creator A5054871862 @default.
- W2078558208 creator A5059159168 @default.
- W2078558208 creator A5068062810 @default.
- W2078558208 creator A5069174677 @default.
- W2078558208 creator A5069690311 @default.
- W2078558208 date "2015-01-11" @default.
- W2078558208 modified "2023-10-17" @default.
- W2078558208 title "A Stable Human-Cell System Overexpressing Cystic Fibrosis Transmembrane Conductance Regulator Recombinant Protein at the Cell Surface" @default.
- W2078558208 cites W120703973 @default.
- W2078558208 cites W1530368384 @default.
- W2078558208 cites W1745204207 @default.
- W2078558208 cites W1769569140 @default.
- W2078558208 cites W1973609285 @default.
- W2078558208 cites W1975971473 @default.
- W2078558208 cites W1976810474 @default.
- W2078558208 cites W1981449360 @default.
- W2078558208 cites W1987760846 @default.
- W2078558208 cites W1998527939 @default.
- W2078558208 cites W1998953032 @default.
- W2078558208 cites W2004477274 @default.
- W2078558208 cites W2005979110 @default.
- W2078558208 cites W2006519535 @default.
- W2078558208 cites W2010863271 @default.
- W2078558208 cites W2013911571 @default.
- W2078558208 cites W2018289835 @default.
- W2078558208 cites W2031948962 @default.
- W2078558208 cites W2039599894 @default.
- W2078558208 cites W2041993512 @default.
- W2078558208 cites W2042079299 @default.
- W2078558208 cites W2049914201 @default.
- W2078558208 cites W2050264979 @default.
- W2078558208 cites W2051189863 @default.
- W2078558208 cites W2052306185 @default.
- W2078558208 cites W2055864348 @default.
- W2078558208 cites W2055894737 @default.
- W2078558208 cites W2058169488 @default.
- W2078558208 cites W2058530636 @default.
- W2078558208 cites W2062402969 @default.
- W2078558208 cites W2062526656 @default.
- W2078558208 cites W2067501477 @default.
- W2078558208 cites W2071973100 @default.
- W2078558208 cites W2081959026 @default.
- W2078558208 cites W2096186501 @default.
- W2078558208 cites W2100837269 @default.
- W2078558208 cites W2108047993 @default.
- W2078558208 cites W2111010372 @default.
- W2078558208 cites W2114995066 @default.
- W2078558208 cites W2115806629 @default.
- W2078558208 cites W2118393797 @default.
- W2078558208 cites W2125071576 @default.
- W2078558208 cites W2127402429 @default.
- W2078558208 cites W2128094872 @default.
- W2078558208 cites W2134703505 @default.
- W2078558208 cites W2141688289 @default.
- W2078558208 cites W2145786361 @default.
- W2078558208 cites W2150712887 @default.
- W2078558208 cites W2150716356 @default.
- W2078558208 cites W2159015766 @default.
- W2078558208 cites W2162746575 @default.
- W2078558208 cites W2165056594 @default.
- W2078558208 cites W2167523976 @default.
- W2078558208 cites W2170989849 @default.
- W2078558208 cites W2171278423 @default.
- W2078558208 cites W2234723228 @default.
- W2078558208 cites W2257974587 @default.
- W2078558208 cites W2289260006 @default.
- W2078558208 cites W2342658150 @default.
- W2078558208 cites W2791163698 @default.
- W2078558208 cites W76071323 @default.
- W2078558208 doi "https://doi.org/10.1007/s12033-014-9830-5" @default.
- W2078558208 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4405497" @default.
- W2078558208 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25808031" @default.
- W2078558208 hasPublicationYear "2015" @default.
- W2078558208 type Work @default.
- W2078558208 sameAs 2078558208 @default.
- W2078558208 citedByCount "22" @default.
- W2078558208 countsByYear W20785582082015 @default.
- W2078558208 countsByYear W20785582082016 @default.
- W2078558208 countsByYear W20785582082017 @default.
- W2078558208 countsByYear W20785582082018 @default.
- W2078558208 countsByYear W20785582082019 @default.
- W2078558208 countsByYear W20785582082020 @default.
- W2078558208 countsByYear W20785582082021 @default.
- W2078558208 countsByYear W20785582082022 @default.
- W2078558208 crossrefType "journal-article" @default.
- W2078558208 hasAuthorship W2078558208A5022159124 @default.