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- W2078558375 abstract "Immunostaining of the cell cycle-associated Ki-67 antigen was studied on routinely formalin-fixed and paraffin-embedded tissue sections, using the Ki-67-specific monoclonal antibody MIB-1. Immunomorphological analysis of the Ki-67 immunostaining pattern was carried out following tissue pre-treatments including combinations of microwave heating and trypsinization, as well as of ribonuclease and deoxyribonuclease pre-digestion of the sections. The nucleolar Ki-67 immunostaining after slide pre-treatment by microwave heating followed by trypsinization was reduced only by ribonuclease pre-digestion, if this latter was used before heating. Ki-67 immunostaining was not significantly reduced by deoxyribonuclease treatment. We conclude that Ki-67, located in the nucleoli, may be associated there with nucleolar RNA." @default.
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- W2078558375 date "1995-02-01" @default.
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- W2078558375 title "Effect of Ribonuclease A and Deoxyribonuclease I on Immunostaining of Ki-67 in Fixed-embedded Sections" @default.
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- W2078558375 doi "https://doi.org/10.1016/s0344-0338(11)80923-4" @default.
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