Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078562842> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2078562842 abstract "Fluorescent probes suitable for the selective detection of DNA sequences are important in genomic research, disease diagnostics, and pathogen detection, among many other applications. The unique optical properties of semiconductor quantum dots (QDs) have proven to be highly valuable for development of fluorescent probes and biosensors. We describe preliminary work toward combining QDs with monomeric thiazole dyes for the detection of nucleic acid hybridization. BO, TO, BO3, and TO3 dyes, which span the visible spectrum, were synthesized with undecanoic acid linkers to permit bioconjugation and their fluorescent enhancements in response to DNA oligonucleotides was evaluated. Contrast ratios between single-stranded probe oligonucleotide and double-stranded probe/target hybrids were between 2.5 and 7.5. BO3 and TO3 were used to label a polyhistidine-appended peptide that self-assembled to QDs and were found to be suitable acceptor dyes for Förster resonance energy transfer (FRET) with QD donors that had their peak emission at 540 nm and 625 nm, respectively. We further conjugated a probe oligonucleotide to a polyhistidineappended peptide at an internal site, and this probe also self-assembled to QDs. Mixing these conjugates with BO3 and either complementary DNA target or non-complementary DNA could induce quenching of the QD emission via FRET, but no FRET-sensitized BO3 emission was observed. Experiments suggested that binding of BO3 to the interface of the QDs was in competition with binding to DNA. Our results provide insight into important criteria (e.g., QD surface chemistry) for designing and optimizing a QD-FRET probe for DNA detection that utilizes the fluorescent properties of monomeric thiazole intercalating dyes." @default.
- W2078562842 created "2016-06-24" @default.
- W2078562842 creator A5012704895 @default.
- W2078562842 creator A5091095826 @default.
- W2078562842 date "2014-05-22" @default.
- W2078562842 modified "2023-09-23" @default.
- W2078562842 title "Evaluation of thiazole intercalating dyes as acceptors for quantum dot donors in Förster resonance energy transfer" @default.
- W2078562842 cites W1986816641 @default.
- W2078562842 cites W1988196812 @default.
- W2078562842 cites W1991646828 @default.
- W2078562842 cites W2003057808 @default.
- W2078562842 cites W2005112585 @default.
- W2078562842 cites W2025042792 @default.
- W2078562842 cites W2026681603 @default.
- W2078562842 cites W2030752820 @default.
- W2078562842 cites W2032860659 @default.
- W2078562842 cites W2034781357 @default.
- W2078562842 cites W2041630712 @default.
- W2078562842 cites W2042480537 @default.
- W2078562842 cites W2043280852 @default.
- W2078562842 cites W2053468384 @default.
- W2078562842 cites W2057153221 @default.
- W2078562842 cites W2060181267 @default.
- W2078562842 cites W2062498920 @default.
- W2078562842 cites W2063170030 @default.
- W2078562842 cites W2066085871 @default.
- W2078562842 cites W2082454728 @default.
- W2078562842 cites W2118950774 @default.
- W2078562842 cites W2166812055 @default.
- W2078562842 cites W2319443826 @default.
- W2078562842 cites W2330622193 @default.
- W2078562842 cites W4230715379 @default.
- W2078562842 cites W4234793439 @default.
- W2078562842 doi "https://doi.org/10.1117/12.2053165" @default.
- W2078562842 hasPublicationYear "2014" @default.
- W2078562842 type Work @default.
- W2078562842 sameAs 2078562842 @default.
- W2078562842 citedByCount "0" @default.
- W2078562842 crossrefType "proceedings-article" @default.
- W2078562842 hasAuthorship W2078562842A5012704895 @default.
- W2078562842 hasAuthorship W2078562842A5091095826 @default.
- W2078562842 hasConcept C120665830 @default.
- W2078562842 hasConcept C121332964 @default.
- W2078562842 hasConcept C124657808 @default.
- W2078562842 hasConcept C129312508 @default.
- W2078562842 hasConcept C160756335 @default.
- W2078562842 hasConcept C171250308 @default.
- W2078562842 hasConcept C185592680 @default.
- W2078562842 hasConcept C192562407 @default.
- W2078562842 hasConcept C23963754 @default.
- W2078562842 hasConcept C2777343594 @default.
- W2078562842 hasConcept C552990157 @default.
- W2078562842 hasConcept C55493867 @default.
- W2078562842 hasConcept C75473681 @default.
- W2078562842 hasConcept C91881484 @default.
- W2078562842 hasConcept C96305047 @default.
- W2078562842 hasConceptScore W2078562842C120665830 @default.
- W2078562842 hasConceptScore W2078562842C121332964 @default.
- W2078562842 hasConceptScore W2078562842C124657808 @default.
- W2078562842 hasConceptScore W2078562842C129312508 @default.
- W2078562842 hasConceptScore W2078562842C160756335 @default.
- W2078562842 hasConceptScore W2078562842C171250308 @default.
- W2078562842 hasConceptScore W2078562842C185592680 @default.
- W2078562842 hasConceptScore W2078562842C192562407 @default.
- W2078562842 hasConceptScore W2078562842C23963754 @default.
- W2078562842 hasConceptScore W2078562842C2777343594 @default.
- W2078562842 hasConceptScore W2078562842C552990157 @default.
- W2078562842 hasConceptScore W2078562842C55493867 @default.
- W2078562842 hasConceptScore W2078562842C75473681 @default.
- W2078562842 hasConceptScore W2078562842C91881484 @default.
- W2078562842 hasConceptScore W2078562842C96305047 @default.
- W2078562842 hasLocation W20785628421 @default.
- W2078562842 hasOpenAccess W2078562842 @default.
- W2078562842 hasPrimaryLocation W20785628421 @default.
- W2078562842 hasRelatedWork W2004255259 @default.
- W2078562842 hasRelatedWork W2012846906 @default.
- W2078562842 hasRelatedWork W2050573321 @default.
- W2078562842 hasRelatedWork W2052561511 @default.
- W2078562842 hasRelatedWork W2068119209 @default.
- W2078562842 hasRelatedWork W2086075014 @default.
- W2078562842 hasRelatedWork W2091750221 @default.
- W2078562842 hasRelatedWork W2120687754 @default.
- W2078562842 hasRelatedWork W3088456700 @default.
- W2078562842 hasRelatedWork W4317892516 @default.
- W2078562842 isParatext "false" @default.
- W2078562842 isRetracted "false" @default.
- W2078562842 magId "2078562842" @default.
- W2078562842 workType "article" @default.