Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078570963> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2078570963 endingPage "e309" @default.
- W2078570963 startingPage "e308" @default.
- W2078570963 abstract "The presence of DNA in the blastocoel provides an attractive target for less invasive aneuploidy screening. However, blastocoel fluid provides many challenges including DNA quantity and integrity. To date, a functional screening algorithm that overcomes these challenges and accurately identifies the karyotype of the blastocyst has not been validated. This study seeks to determine consistency of diagnosis between DNA isolated from trophectoderm and blastocoel fluid using NextGen based CCS. Prospective blinded. Embryos diagnosed as aneuploid by CCS following trophectoderm biopsy (TE Bx) had their blastocoel fluid aspirated using a standard microinjection pipette. Whole genome amplification (WGA) and NextGen sequencing was performed using a protocol validated on TE Bx’s. Aneuploidy screening results from the blastocoel were then compared to results obtained from TE Bx’s to determine concordance levels. The amplification efficiency of the blastocoel sample and sequencing depth were also compared to see if they prognosticated the accuracy of the result. Forty nine embryos with a wide variety of chromosomal aneuploidies were studied. WGA was successful in 47/49 (96%) samples. Of those, 13 (28%) gave a consistent diagnosis, which exactly matched the TE Bx result. An additional 4 (9%) samples predicted at least one aneuploid chromosome correctly, but did not completely match the original result. WGA yield (p=0.003) and sequencing depth (p=0.008) were higher in samples with results identical to those attained in the TE Bx’s. Samples with depths below 4400 were consistently inaccurate. Those with > 36,000 reads were correct in 12 of 13 samples. This study demonstrates that the accuracy of CCS from blastocoel fluid is dependent on the amplification efficiency and the total reads attained through NextGen sequencing. This algorithm is not reliable at this time. Can the efficiency be improved by enhancing the analytical algorithm? Or do low yield samples represent a biologic limit reflecting the amount of intact DNA in the fluid? Progress is being made but further investigation is required. Direct testing through trophectoderm biopsy remains the optimum methodology for safely and effectively applying CCS technology at this time." @default.
- W2078570963 created "2016-06-24" @default.
- W2078570963 creator A5009008306 @default.
- W2078570963 creator A5010519012 @default.
- W2078570963 creator A5011160568 @default.
- W2078570963 creator A5035392494 @default.
- W2078570963 creator A5047100994 @default.
- W2078570963 creator A5048845992 @default.
- W2078570963 creator A5068218784 @default.
- W2078570963 creator A5079083254 @default.
- W2078570963 creator A5079214360 @default.
- W2078570963 date "2014-09-01" @default.
- W2078570963 modified "2023-09-29" @default.
- W2078570963 title "The accuracy of blastocoel fluid comprehensive chromosomal screening (CCS) is dependent on amplification yield and sequencing depth when using nextgen sequencing" @default.
- W2078570963 doi "https://doi.org/10.1016/j.fertnstert.2014.07.1048" @default.
- W2078570963 hasPublicationYear "2014" @default.
- W2078570963 type Work @default.
- W2078570963 sameAs 2078570963 @default.
- W2078570963 citedByCount "2" @default.
- W2078570963 countsByYear W20785709632018 @default.
- W2078570963 countsByYear W20785709632020 @default.
- W2078570963 crossrefType "journal-article" @default.
- W2078570963 hasAuthorship W2078570963A5009008306 @default.
- W2078570963 hasAuthorship W2078570963A5010519012 @default.
- W2078570963 hasAuthorship W2078570963A5011160568 @default.
- W2078570963 hasAuthorship W2078570963A5035392494 @default.
- W2078570963 hasAuthorship W2078570963A5047100994 @default.
- W2078570963 hasAuthorship W2078570963A5048845992 @default.
- W2078570963 hasAuthorship W2078570963A5068218784 @default.
- W2078570963 hasAuthorship W2078570963A5079083254 @default.
- W2078570963 hasAuthorship W2078570963A5079214360 @default.
- W2078570963 hasBestOaLocation W20785709631 @default.
- W2078570963 hasConcept C104317684 @default.
- W2078570963 hasConcept C147789679 @default.
- W2078570963 hasConcept C151552171 @default.
- W2078570963 hasConcept C160798450 @default.
- W2078570963 hasConcept C185592680 @default.
- W2078570963 hasConcept C196843134 @default.
- W2078570963 hasConcept C2777804106 @default.
- W2078570963 hasConcept C2778177303 @default.
- W2078570963 hasConcept C2779672484 @default.
- W2078570963 hasConcept C30481170 @default.
- W2078570963 hasConcept C51679486 @default.
- W2078570963 hasConcept C54355233 @default.
- W2078570963 hasConcept C552990157 @default.
- W2078570963 hasConcept C70721500 @default.
- W2078570963 hasConcept C86803240 @default.
- W2078570963 hasConcept C87073359 @default.
- W2078570963 hasConceptScore W2078570963C104317684 @default.
- W2078570963 hasConceptScore W2078570963C147789679 @default.
- W2078570963 hasConceptScore W2078570963C151552171 @default.
- W2078570963 hasConceptScore W2078570963C160798450 @default.
- W2078570963 hasConceptScore W2078570963C185592680 @default.
- W2078570963 hasConceptScore W2078570963C196843134 @default.
- W2078570963 hasConceptScore W2078570963C2777804106 @default.
- W2078570963 hasConceptScore W2078570963C2778177303 @default.
- W2078570963 hasConceptScore W2078570963C2779672484 @default.
- W2078570963 hasConceptScore W2078570963C30481170 @default.
- W2078570963 hasConceptScore W2078570963C51679486 @default.
- W2078570963 hasConceptScore W2078570963C54355233 @default.
- W2078570963 hasConceptScore W2078570963C552990157 @default.
- W2078570963 hasConceptScore W2078570963C70721500 @default.
- W2078570963 hasConceptScore W2078570963C86803240 @default.
- W2078570963 hasConceptScore W2078570963C87073359 @default.
- W2078570963 hasIssue "3" @default.
- W2078570963 hasLocation W20785709631 @default.
- W2078570963 hasOpenAccess W2078570963 @default.
- W2078570963 hasPrimaryLocation W20785709631 @default.
- W2078570963 hasRelatedWork W2017111986 @default.
- W2078570963 hasRelatedWork W2078570963 @default.
- W2078570963 hasRelatedWork W2809519566 @default.
- W2078570963 hasRelatedWork W2901190885 @default.
- W2078570963 hasRelatedWork W2950219943 @default.
- W2078570963 hasRelatedWork W3023193177 @default.
- W2078570963 hasRelatedWork W3093103135 @default.
- W2078570963 hasRelatedWork W3199781910 @default.
- W2078570963 hasRelatedWork W4246396844 @default.
- W2078570963 hasRelatedWork W4299879818 @default.
- W2078570963 hasVolume "102" @default.
- W2078570963 isParatext "false" @default.
- W2078570963 isRetracted "false" @default.
- W2078570963 magId "2078570963" @default.
- W2078570963 workType "article" @default.