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- W2078596567 abstract "DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 73:201-205 (2007) - doi:10.3354/dao073201 Detection of nodavirus in seawater from rearing facilities for Atlantic halibut Hippoglossus hippoglossus larvae Audun H. Nerland1,*, Cecilie Skaar1, Tove Boge Eriksen1, Hogne Bleie2 1Institute of Marine Research, PO Box 1870 Nordnes, 5817 Bergen, Norway 2National Veterinary Institute, PO Box 1263 Sentrum, 5811 Bergen, Norway *Email: audun.nerland@imr.no ABSTRACT: We used (1) ultracentrifugation followed by RT-PCR and (2) real-time RT-PCR to detect and quantify nodaviruses in seawater in which Atlantic halibut Hippoglossus hippoglossus larvae/fry had been held at rearing facilities. Evaluated against in vitro propagated viruses, the viral concentration corresponded to 1.6 × 104 TCID50 (50% tissue culture infectious dose) ml1. Evaluated against in vitro transcribed RNA, the concentration was estimated at 2 × 107 virus particles ml1 seawater. KEY WORDS: Nodavirus · RT-PCR · Real-time PCR · Quantification · Seawater · Atlantic halibut Full text in pdf format PreviousNextExport citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 73, No. 3. Online publication date: January 18, 2007 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2007 Inter-Research." @default.
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- W2078596567 date "2007-01-18" @default.
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- W2078596567 title "Detection of nodavirus in seawater from rearing facilities for Atlantic halibut Hippoglossus hippoglossus larvae" @default.
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- W2078596567 doi "https://doi.org/10.3354/dao073201" @default.
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