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- W2078598214 abstract "Modern automated microscopic imaging techniques such as high-content screening (HCS), high-throughputscreening, 4D imaging, and multispectral imaging are capable of producing hundreds to thousands of imagesper experiment. For quick retrieval, fast transmission, and storage economy, these images should be saved ina compressed format. A considerable number of techniques based on interband and intraband redundancies ofmultispectral images have been proposed in the literature for the compression of multispectral and 3D temporaldata. However, these works have been carried out mostly in the elds of remote sensing and video processing.Compression for multispectral optical microscopy imaging, with its own set of specialized requirements, hasremained under-investigated. Digital photography{oriented 2D compression techniques like JPEG (ISO/IECIS 10918-1) and JPEG2000 (ISO/IEC 15444-1) are generally adopted for multispectral images which optimizevisual quality but do not necessarily preserve the integrity of scientic data, not to mention the suboptimalperformance of 2D compression techniques in compressing 3D images.Herein we report our work on a new low bit-rate wavelet-based compression scheme for multispectral fluorescencebiological imaging. The sparsity of signicant coefficients in high-frequency subbands of multispectralmicroscopic images is found to be much greater than in natural images; therefore a quad-tree concept such asSaid et al.'s SPIHT1 along with correlation of insignicant wavelet coefficients has been proposed to furtherexploit redundancy at high-frequency subbands. Our work propose a 3D extension to SPIHT, incorporating anew hierarchal inter- and intra-spectral relationship amongst the coefficients of 3D wavelet-decomposed image.The new relationship, apart from adopting the parent-child relationship of classical SPIHT, also brought forththe conditional sibling relationship by relating only the insignicant wavelet coefficients of subbands at thesame level of decomposition. The insignicant quadtrees in dierent subbands in the high-frequency subbandclass are coded by a combined function to reduce redundancy. A number of experiments conducted on microscopicmultispectral images have shown promising results for the proposed method over current state-of-the-artimage-compression techniques." @default.
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- W2078598214 date "2009-02-05" @default.
- W2078598214 modified "2023-09-23" @default.
- W2078598214 title "Compression of multispectral fluorescence microscopic images based on a modified set partitioning in hierarchal trees" @default.
- W2078598214 doi "https://doi.org/10.1117/12.806038" @default.
- W2078598214 hasPublicationYear "2009" @default.
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