Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078602922> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2078602922 endingPage "7" @default.
- W2078602922 startingPage "1" @default.
- W2078602922 abstract "There is great interest in achieving reproducibly high survivals of mammalian oocytes (especially human) after cryopreservation, but the results to date have not matched the interest. A prime cause of cell death is the formation of more than trace amounts of intracellular ice, and one strategy to avoid it is vitrification. In vitrification procedures, cells are loaded with high concentrations of glass-inducing solutes and cooled to -196°C at rates high enough to presumably induce the glassy state. In the last decade, several devices have been developed to achieve very high cooling rates. Nearly all in the field have assumed that the cooling rate is the critical factor. The purpose of our study was to test that assumption by examining the consequences of cooling mouse oocytes in a vitrification solution at four rates ranging from 95 to 69,250°C/min to -196°C and for each cooling rate, subjecting them to five warming rates back above 0°C at rates ranging from 610 to 118,000°C/min. In samples warmed at the highest rate (118,000°C/min), survivals were 70% to 85% regardless of the prior cooling rate. In samples warmed at the lowest rate (610°C/min), survivals were low regardless of the prior cooling rate, but decreased from 25% to 0% as the cooling rate was increased from 95 to 69,000°C/min. Intermediate cooling and warming rates gave intermediate survivals. The especially high sensitivity of survival to warming rate suggests that either the crystallization of intracellular glass during warming or the growth by recrystallization of small intracellular ice crystals formed during cooling are responsible for the lethality of slow warming." @default.
- W2078602922 created "2016-06-24" @default.
- W2078602922 creator A5026970682 @default.
- W2078602922 creator A5038468938 @default.
- W2078602922 date "2011-02-01" @default.
- W2078602922 modified "2023-10-17" @default.
- W2078602922 title "Survival of mouse oocytes after being cooled in a vitrification solution to −196°C at 95° to 70,000°C/min and warmed at 610° to 118,000°C/min: A new paradigm for cryopreservation by vitrification" @default.
- W2078602922 cites W1965764259 @default.
- W2078602922 cites W1983588838 @default.
- W2078602922 cites W1996509279 @default.
- W2078602922 cites W1997386840 @default.
- W2078602922 cites W2024988815 @default.
- W2078602922 cites W2025697799 @default.
- W2078602922 cites W2027818414 @default.
- W2078602922 cites W2036594948 @default.
- W2078602922 cites W2048332595 @default.
- W2078602922 cites W2049978882 @default.
- W2078602922 cites W2064297433 @default.
- W2078602922 cites W2077719186 @default.
- W2078602922 cites W2083862421 @default.
- W2078602922 cites W2084423453 @default.
- W2078602922 cites W2084529284 @default.
- W2078602922 cites W2086474578 @default.
- W2078602922 cites W2110462805 @default.
- W2078602922 cites W2117567356 @default.
- W2078602922 cites W2145780187 @default.
- W2078602922 doi "https://doi.org/10.1016/j.cryobiol.2010.10.159" @default.
- W2078602922 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3041861" @default.
- W2078602922 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21055397" @default.
- W2078602922 hasPublicationYear "2011" @default.
- W2078602922 type Work @default.
- W2078602922 sameAs 2078602922 @default.
- W2078602922 citedByCount "143" @default.
- W2078602922 countsByYear W20786029222012 @default.
- W2078602922 countsByYear W20786029222013 @default.
- W2078602922 countsByYear W20786029222014 @default.
- W2078602922 countsByYear W20786029222015 @default.
- W2078602922 countsByYear W20786029222016 @default.
- W2078602922 countsByYear W20786029222017 @default.
- W2078602922 countsByYear W20786029222018 @default.
- W2078602922 countsByYear W20786029222019 @default.
- W2078602922 countsByYear W20786029222020 @default.
- W2078602922 countsByYear W20786029222021 @default.
- W2078602922 countsByYear W20786029222022 @default.
- W2078602922 countsByYear W20786029222023 @default.
- W2078602922 crossrefType "journal-article" @default.
- W2078602922 hasAuthorship W2078602922A5026970682 @default.
- W2078602922 hasAuthorship W2078602922A5038468938 @default.
- W2078602922 hasBestOaLocation W20786029222 @default.
- W2078602922 hasConcept C140793950 @default.
- W2078602922 hasConcept C16685009 @default.
- W2078602922 hasConcept C179933525 @default.
- W2078602922 hasConcept C185592680 @default.
- W2078602922 hasConcept C192562407 @default.
- W2078602922 hasConcept C196843134 @default.
- W2078602922 hasConcept C2778022349 @default.
- W2078602922 hasConcept C71924100 @default.
- W2078602922 hasConcept C86803240 @default.
- W2078602922 hasConcept C95444343 @default.
- W2078602922 hasConceptScore W2078602922C140793950 @default.
- W2078602922 hasConceptScore W2078602922C16685009 @default.
- W2078602922 hasConceptScore W2078602922C179933525 @default.
- W2078602922 hasConceptScore W2078602922C185592680 @default.
- W2078602922 hasConceptScore W2078602922C192562407 @default.
- W2078602922 hasConceptScore W2078602922C196843134 @default.
- W2078602922 hasConceptScore W2078602922C2778022349 @default.
- W2078602922 hasConceptScore W2078602922C71924100 @default.
- W2078602922 hasConceptScore W2078602922C86803240 @default.
- W2078602922 hasConceptScore W2078602922C95444343 @default.
- W2078602922 hasIssue "1" @default.
- W2078602922 hasLocation W20786029221 @default.
- W2078602922 hasLocation W20786029222 @default.
- W2078602922 hasLocation W20786029223 @default.
- W2078602922 hasLocation W20786029224 @default.
- W2078602922 hasOpenAccess W2078602922 @default.
- W2078602922 hasPrimaryLocation W20786029221 @default.
- W2078602922 hasRelatedWork W2001959059 @default.
- W2078602922 hasRelatedWork W2005835660 @default.
- W2078602922 hasRelatedWork W2013827922 @default.
- W2078602922 hasRelatedWork W2058319711 @default.
- W2078602922 hasRelatedWork W2141490755 @default.
- W2078602922 hasRelatedWork W2253749132 @default.
- W2078602922 hasRelatedWork W2372652522 @default.
- W2078602922 hasRelatedWork W2388834060 @default.
- W2078602922 hasRelatedWork W2582653153 @default.
- W2078602922 hasRelatedWork W2944695536 @default.
- W2078602922 hasVolume "62" @default.
- W2078602922 isParatext "false" @default.
- W2078602922 isRetracted "false" @default.
- W2078602922 magId "2078602922" @default.
- W2078602922 workType "article" @default.