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- W2078605979 abstract "In the present study, an Enterococcus-Escherichia coli shuttle vector, pC3, was constructed that allows efficient transformation by electroporation of Enterococcus hirae ATCC9790. 5 x 10(6) transformants per microgram of plasmid DNA were obtained, using a commercial capacitor discharge device with an improved circuitry and a home-made electrode assembly, delivering pulses of 24 kV/cm across the cell suspension. The transformants were stable without selective pressure and plasmid DNA reisolated from transformed cells displayed no alterations in restriction enzyme analysis. Chromosomal DNA from E coli or E hirae, carried by pC3, was stably maintained in E hirae, making cloning and genetic manipulation in this organism feasible." @default.
- W2078605979 created "2016-06-24" @default.
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- W2078605979 date "1990-04-01" @default.
- W2078605979 modified "2023-09-23" @default.
- W2078605979 title "Efficient electrotransformation of Enterococcus hirae with a new Enterococcus-Escherichia coli shuttle vector" @default.
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- W2078605979 doi "https://doi.org/10.1016/0300-9084(90)90084-t" @default.
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