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- W2078607325 abstract "The aim of this study was to define the effects of a potent inhibitor of tyrosine phosphatases, sodium orthovanadate (0.1-100 microM for up to 48 h), on dentate gyrus cells (DGC) in culture. Treatment with 100 microM orthovanadate evoked a delayed form of cell death. To examine the possible involvement of apoptosis in orthovanadate-induced cell death, biochemical and morphological alterations were compared with those of necrotic death induced by sodium azide. Phase-contrast microscopy and nuclear condensation analysis showed that orthovanadate and azide each evoked cell death by distinct pathways. TUNEL assay was positive in both cases. Application of a protein synthesis inhibitor, cycloheximide, did not prevent cytotoxicity caused by either orthovanadate or azide and potentiated the effects of vanadate. We conclude that orthovanadate-induced death of DGC bears features of apoptosis." @default.
- W2078607325 created "2016-06-24" @default.
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- W2078607325 date "1997-07-01" @default.
- W2078607325 modified "2023-09-25" @default.
- W2078607325 title "Orthovanadate induces cell death in rat dentate gyrus primary culture" @default.
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- W2078607325 doi "https://doi.org/10.1097/00001756-199707280-00011" @default.
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