Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078607774> ?p ?o ?g. }
- W2078607774 endingPage "479" @default.
- W2078607774 startingPage "473" @default.
- W2078607774 abstract "Purpose To establish a clinically applicable protocol for quantification of residual γH2AX foci in ex vivo irradiated tumour samples and to apply this method in a proof-of-concept feasibility study to patient-derived tumour specimens. Material and methods Evaluation of γH2AX foci formation and disappearance in excised FaDu tumour specimens after (a) different incubation times in culture medium, 4 Gy irradiation and fixation after 24 h (cell recovery), (b) 10 h medium incubation, 4 Gy irradiation and fixation after various time points (double strand break repair kinetics), and (c) 10 h medium incubation, irradiation with graded single radiation doses and fixation after 24 h (dose–response). The optimised protocol was applied to patient-derived samples of seminoma, prostate cancer and glioblastoma multiforme. Results Post excision or biopsy, tumour tissues showed stable radiation-induced γH2AX foci values in oxic cells after >6 h of recovery in medium. Kinetics of foci disappearance indicated a plateau of residual foci after >12 h following ex vivo irradiation. Fitting the dose–response of residual γH2AX foci yielded slopes comparable with in situ irradiation of FaDu tumours. Significant differences in the slopes of ex vivo irradiated patient-derived tumour samples were found. Conclusion A novel clinically applicable method to quantify residual γH2AX foci in ex vivo irradiated tumour samples was established. The first clinical results suggest that this method allows to distinguish between radiosensitive and radioresistant tumour types. These findings support further translational evaluation of this assay to individualise radiation therapy." @default.
- W2078607774 created "2016-06-24" @default.
- W2078607774 creator A5002463670 @default.
- W2078607774 creator A5012745984 @default.
- W2078607774 creator A5015359720 @default.
- W2078607774 creator A5027241562 @default.
- W2078607774 creator A5028492548 @default.
- W2078607774 creator A5030310687 @default.
- W2078607774 creator A5037651119 @default.
- W2078607774 creator A5066620282 @default.
- W2078607774 creator A5070431570 @default.
- W2078607774 creator A5079383491 @default.
- W2078607774 creator A5083101824 @default.
- W2078607774 date "2015-09-01" @default.
- W2078607774 modified "2023-10-17" @default.
- W2078607774 title "γH2AX assay in ex vivo irradiated tumour specimens: A novel method to determine tumour radiation sensitivity in patient-derived material" @default.
- W2078607774 cites W1568605026 @default.
- W2078607774 cites W1963861821 @default.
- W2078607774 cites W1964254408 @default.
- W2078607774 cites W1971096822 @default.
- W2078607774 cites W1985775217 @default.
- W2078607774 cites W1986351585 @default.
- W2078607774 cites W2005459546 @default.
- W2078607774 cites W2012126331 @default.
- W2078607774 cites W2012801703 @default.
- W2078607774 cites W2014478946 @default.
- W2078607774 cites W2023467037 @default.
- W2078607774 cites W2025633794 @default.
- W2078607774 cites W2027630134 @default.
- W2078607774 cites W2032980832 @default.
- W2078607774 cites W2034277347 @default.
- W2078607774 cites W2042830951 @default.
- W2078607774 cites W2051003586 @default.
- W2078607774 cites W2051193631 @default.
- W2078607774 cites W2055907804 @default.
- W2078607774 cites W2059442193 @default.
- W2078607774 cites W2061414735 @default.
- W2078607774 cites W2068727486 @default.
- W2078607774 cites W2070582215 @default.
- W2078607774 cites W2075758387 @default.
- W2078607774 cites W2077892030 @default.
- W2078607774 cites W2083875981 @default.
- W2078607774 cites W2085362274 @default.
- W2078607774 cites W2087508922 @default.
- W2078607774 cites W2089519759 @default.
- W2078607774 cites W2091136099 @default.
- W2078607774 cites W2096136751 @default.
- W2078607774 cites W2099254999 @default.
- W2078607774 cites W2102173037 @default.
- W2078607774 cites W2102560312 @default.
- W2078607774 cites W2106195394 @default.
- W2078607774 cites W2114584615 @default.
- W2078607774 cites W2117189250 @default.
- W2078607774 cites W2130883309 @default.
- W2078607774 cites W2131661783 @default.
- W2078607774 cites W2138242349 @default.
- W2078607774 cites W2142460487 @default.
- W2078607774 cites W2158681922 @default.
- W2078607774 cites W2160407692 @default.
- W2078607774 cites W4234187335 @default.
- W2078607774 doi "https://doi.org/10.1016/j.radonc.2015.03.026" @default.
- W2078607774 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25866027" @default.
- W2078607774 hasPublicationYear "2015" @default.
- W2078607774 type Work @default.
- W2078607774 sameAs 2078607774 @default.
- W2078607774 citedByCount "36" @default.
- W2078607774 countsByYear W20786077742015 @default.
- W2078607774 countsByYear W20786077742016 @default.
- W2078607774 countsByYear W20786077742017 @default.
- W2078607774 countsByYear W20786077742018 @default.
- W2078607774 countsByYear W20786077742019 @default.
- W2078607774 countsByYear W20786077742020 @default.
- W2078607774 countsByYear W20786077742021 @default.
- W2078607774 countsByYear W20786077742022 @default.
- W2078607774 countsByYear W20786077742023 @default.
- W2078607774 crossrefType "journal-article" @default.
- W2078607774 hasAuthorship W2078607774A5002463670 @default.
- W2078607774 hasAuthorship W2078607774A5012745984 @default.
- W2078607774 hasAuthorship W2078607774A5015359720 @default.
- W2078607774 hasAuthorship W2078607774A5027241562 @default.
- W2078607774 hasAuthorship W2078607774A5028492548 @default.
- W2078607774 hasAuthorship W2078607774A5030310687 @default.
- W2078607774 hasAuthorship W2078607774A5037651119 @default.
- W2078607774 hasAuthorship W2078607774A5066620282 @default.
- W2078607774 hasAuthorship W2078607774A5070431570 @default.
- W2078607774 hasAuthorship W2078607774A5079383491 @default.
- W2078607774 hasAuthorship W2078607774A5083101824 @default.
- W2078607774 hasConcept C111337013 @default.
- W2078607774 hasConcept C121332964 @default.
- W2078607774 hasConcept C126838900 @default.
- W2078607774 hasConcept C142724271 @default.
- W2078607774 hasConcept C146249460 @default.
- W2078607774 hasConcept C150903083 @default.
- W2078607774 hasConcept C185544564 @default.
- W2078607774 hasConcept C185592680 @default.
- W2078607774 hasConcept C207001950 @default.
- W2078607774 hasConcept C26291073 @default.
- W2078607774 hasConcept C2775934546 @default.