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- W2078673312 abstract "The presence of optimal levels of water in enzymatic hydrolysis reactions in a supercritical CO2 (SCCO2) medium is critical not only as a reactant but also in order to maintain the catalytic activity of the enzyme. The effects of the water flow rate (0.002−0.050 mL/min), the amounts of canola flake (3.0 and 15.0 g) and enzyme (1.0 and 5.0 g of immobilized lipase from Mucor miehei, Lipozyme IM) loads, and the reaction cell size were studied in the continuous enzymatic hydrolysis of canola oil in SCCO2 at 24 MPa and 35 °C using an online extraction−reaction system. Supercritical fluid chromatography was used to analyze the product mixtures for their free fatty acid (FFA) and mono-, di-, and triglyceride contents. At the water flow rate of 0.002 mL/min, 97% triglyceride conversion was achieved, i.e., almost a complete hydrolysis. High levels of FFA in the product were obtained with 3 g of canola load, using a smaller reaction cell, and 5 g of enzyme load, indicating a greater extent of hydrolysis." @default.
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- W2078673312 date "2002-11-12" @default.
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- W2078673312 title "Effect of Water on Canola Oil Hydrolysis in an Online Extraction−Reaction System Using Supercritical CO<sub>2</sub>" @default.
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- W2078673312 doi "https://doi.org/10.1021/ie0201359" @default.
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