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• W2078689672 abstract "An attractive option to produce chiral amines of industrial importance is through asymmetric synthesis using ω-transaminase. However, reaching high yields often requires a strategy for shifting the equilibrium position. This paper describes a novel strategy for handling this problem. It involves the use of a supported liquid membrane (SLM) together with a packed bed reactor. The reactor contains Escherichia coli cells with ω-transaminase from Arthrobacter citreus, immobilized by flocculation with chitosan. The SLM consists of a hollow fibre membrane contactor in which the pores contain undecane. The system enables continuous extraction of the amine product and was used to successfully shift the equilibrium in asymmetric synthesis of (S)-α-methylbenzylamine (MBA). A conversion of 98% was reached, compared to 50% without product extraction. Moreover, a selective extraction of the produced MBA was realized. A high product concentration of 55 g/l was reached after 80 h, and the system showed promising potential for continuous operation." @default.
• W2078689672 created "2016-06-24" @default.
• W2078689672 creator A5047896793 @default.
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• W2078689672 date "2014-06-01" @default.
• W2078689672 modified "2023-09-26" @default.
• W2078689672 title "Supported liquid membrane as a novel tool for driving the equilibrium of ω-transaminase catalyzed asymmetric synthesis" @default.
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• W2078689672 doi "https://doi.org/10.1016/j.jbiotec.2014.03.022" @default.
• W2078689672 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24675224" @default.
• W2078689672 hasPublicationYear "2014" @default.
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