FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078696541> ?p ?o ?g. }

• W2078696541 abstract "Biofilm formation is a general attribute to almost all bacteria( 1-6). When bacteria form biofilms, cells are encased in extracellular matrix that is mostly constituted by proteins and exopolysaccharides, among other factors (7-10). The microbial community encased within the biofilm often shows the differentiation of distinct subpopulation of specialized cells (11-17). These subpopulations coexist and often show spatial and temporal organization within the biofilm ( 18-21). Biofilm formation in the model organism Bacillus subtilis requires the differentiation of distinct subpopulations of specialized cells. Among them, the subpopulation of matrix producers, responsible to produce and secrete the extracellular matrix of the biofilm is essential for biofilm formation (11,19). Hence, differentiation of matrix producers is a hallmark of biofilm formation in B. subtilis. We have used fluorescent reporters to visualize and quantify the subpopulation of matrix producers in biofilms of B. subtilis (15,19,22-24). Concretely, we have observed that the subpopulation of matrix producers differentiates in response to the presence of self-produced extracellular signal surfactin (25). Interestingly, surfactin is produced by a subpopulation of specialized cells different from the subpopulation of matrix producers (15). We have detailed in this report the technical approach necessary to visualize and quantify the subpopulation of matrix producers and surfactin producers within the biofilms of B. subtilis. To do this, fluorescent reporters of genes required for matrix production and surfactin production are inserted into the chromosome of B. subtilis. Reporters are expressed only in a subpopulation of specialized cells. Then, the subpopulations can be monitored using fluorescence microscopy and flow cytometry (See Fig 1). The fact that different subpopulations of specialized cells coexist within multicellular communities of bacteria gives us a different perspective about the regulation of gene expression in prokaryotes. This protocol addresses this phenomenon experimentally and it can be easily adapted to any other working model, to elucidate the molecular mechanisms underlying phenotypic heterogeneity within a microbial community." @default.
• W2078696541 created "2016-06-24" @default.
• W2078696541 creator A5004226170 @default.
• W2078696541 creator A5028058766 @default.
• W2078696541 creator A5028160852 @default.
• W2078696541 creator A5079848082 @default.
• W2078696541 date "2012-02-15" @default.
• W2078696541 modified "2023-09-25" @default.
• W2078696541 title "Single-cell Analysis of <em>Bacillus subtilis</em> Biofilms Using Fluorescence Microscopy and Flow Cytometry" @default.
• W2078696541 cites W1503194715 @default.
• W2078696541 cites W1515668124 @default.
• W2078696541 cites W1676315537 @default.
• W2078696541 cites W1758794665 @default.
• W2078696541 cites W1956487875 @default.
• W2078696541 cites W1993670200 @default.
• W2078696541 cites W1996064131 @default.
• W2078696541 cites W2020496125 @default.
• W2078696541 cites W2030148834 @default.
• W2078696541 cites W2039434324 @default.
• W2078696541 cites W2047003155 @default.
• W2078696541 cites W2084524312 @default.
• W2078696541 cites W2099487698 @default.
• W2078696541 cites W2102838174 @default.
• W2078696541 cites W2106503480 @default.
• W2078696541 cites W2107962506 @default.
• W2078696541 cites W2110324548 @default.
• W2078696541 cites W2111899305 @default.
• W2078696541 cites W2120978086 @default.
• W2078696541 cites W2124980889 @default.
• W2078696541 cites W2133397415 @default.
• W2078696541 cites W2134495017 @default.
• W2078696541 cites W2143027164 @default.
• W2078696541 cites W2143768216 @default.
• W2078696541 cites W2144821695 @default.
• W2078696541 cites W2149134207 @default.
• W2078696541 cites W2149886824 @default.
• W2078696541 cites W2152042119 @default.
• W2078696541 cites W2163288778 @default.
• W2078696541 cites W2163463398 @default.
• W2078696541 cites W2163970869 @default.
• W2078696541 cites W2168403192 @default.
• W2078696541 cites W2171397200 @default.
• W2078696541 doi "https://doi.org/10.3791/3796" @default.
• W2078696541 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3376938" @default.
• W2078696541 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22371091" @default.
• W2078696541 hasPublicationYear "2012" @default.
• W2078696541 type Work @default.
• W2078696541 sameAs 2078696541 @default.
• W2078696541 citedByCount "24" @default.
• W2078696541 countsByYear W20786965412012 @default.
• W2078696541 countsByYear W20786965412013 @default.
• W2078696541 countsByYear W20786965412014 @default.
• W2078696541 countsByYear W20786965412015 @default.
• W2078696541 countsByYear W20786965412016 @default.
• W2078696541 countsByYear W20786965412017 @default.
• W2078696541 countsByYear W20786965412018 @default.
• W2078696541 countsByYear W20786965412019 @default.
• W2078696541 countsByYear W20786965412020 @default.
• W2078696541 countsByYear W20786965412021 @default.
• W2078696541 countsByYear W20786965412022 @default.
• W2078696541 countsByYear W20786965412023 @default.
• W2078696541 crossrefType "journal-article" @default.
• W2078696541 hasAuthorship W2078696541A5004226170 @default.
• W2078696541 hasAuthorship W2078696541A5028058766 @default.
• W2078696541 hasAuthorship W2078696541A5028160852 @default.
• W2078696541 hasAuthorship W2078696541A5079848082 @default.
• W2078696541 hasBestOaLocation W20786965412 @default.
• W2078696541 hasConcept C106487976 @default.
• W2078696541 hasConcept C121332964 @default.
• W2078696541 hasConcept C153911025 @default.
• W2078696541 hasConcept C169274487 @default.
• W2078696541 hasConcept C185592680 @default.
• W2078696541 hasConcept C189165786 @default.
• W2078696541 hasConcept C2776102837 @default.
• W2078696541 hasConcept C2777272437 @default.
• W2078696541 hasConcept C28406088 @default.
• W2078696541 hasConcept C43617362 @default.
• W2078696541 hasConcept C523546767 @default.
• W2078696541 hasConcept C54355233 @default.
• W2078696541 hasConcept C553184892 @default.
• W2078696541 hasConcept C58123911 @default.
• W2078696541 hasConcept C62520636 @default.
• W2078696541 hasConcept C86803240 @default.
• W2078696541 hasConcept C89423630 @default.
• W2078696541 hasConcept C91881484 @default.
• W2078696541 hasConcept C95444343 @default.
• W2078696541 hasConceptScore W2078696541C106487976 @default.
• W2078696541 hasConceptScore W2078696541C121332964 @default.
• W2078696541 hasConceptScore W2078696541C153911025 @default.
• W2078696541 hasConceptScore W2078696541C169274487 @default.
• W2078696541 hasConceptScore W2078696541C185592680 @default.
• W2078696541 hasConceptScore W2078696541C189165786 @default.
• W2078696541 hasConceptScore W2078696541C2776102837 @default.
• W2078696541 hasConceptScore W2078696541C2777272437 @default.
• W2078696541 hasConceptScore W2078696541C28406088 @default.
• W2078696541 hasConceptScore W2078696541C43617362 @default.
• W2078696541 hasConceptScore W2078696541C523546767 @default.
• W2078696541 hasConceptScore W2078696541C54355233 @default.
• W2078696541 hasConceptScore W2078696541C553184892 @default.
• W2078696541 hasConceptScore W2078696541C58123911 @default.

• next