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- W2078713908 abstract "The covalent flavin attachment site in the Arthrobacter sarcosine oxidase (cysteine at position 318) was replaced with serine, and the mutational effect of C318S was analyzed. Wild type and C318S with a C-terminal 6-histidine tag were constructed and homogeneously purified by the single step. The covalently binding to flavin was not essential to the enzyme activity because the C318S mutant exhibited extremely weak activity. Moreover, the activity of the mutant was recovered in the presence of flavin adenine dinucleotide (FAD), and significantly increased as the concentration of FAD increased. This dependence of the mutant on FAD indicates that the noncovalent binding of free FAD to the mutant enzyme is reversible." @default.
- W2078713908 created "2016-06-24" @default.
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- W2078713908 date "2000-10-01" @default.
- W2078713908 modified "2023-09-27" @default.
- W2078713908 title "A Mutant Sarcosine Oxidase in Which Activity Depends on Flavin Adenine Dinucleotide" @default.
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- W2078713908 doi "https://doi.org/10.1006/prep.2000.1299" @default.
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