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- W2078717569 abstract "Transcription pausing is a key step in many prokaryotic transcription attenuation mechanisms. Pausing is thought to occur when an RNA hairpin forms near the 3' end of a growing transcript. We report here the isolation of the trp leader paused transcription complex containing a defined 92-nucleotide nascent transcript. Digestion of isolated paused complexes with RNase T1 suggests that the trp leader RNA hairpin designated 1:2 forms in the paused transcription complex. The transcription factor NusA alters the RNase T1 digestion pattern of the 92-nucleotide pause transcript in the complex but not the cleavage patterns of purified pause RNA, suggesting that NusA specifically affects the 1:2 hairpin in the paused transcription complex. The isolated paused transcription complex retains the ability to resume transcription. Kinetic studies on the resumption of elongation suggest that NusA is a non-competitive inhibitor of paused complex release and that the Ks for GTP is around 300 microM. RNA polymerase in the paused transcription complex protects approximately 30 base-pairs on both DNA strands from exonuclease digestion." @default.
- W2078717569 created "2016-06-24" @default.
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- W2078717569 date "1987-07-01" @default.
- W2078717569 modified "2023-09-26" @default.
- W2078717569 title "Isolation and structural analysis of the Escherichia coli trp leader paused transcription complex" @default.
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- W2078717569 doi "https://doi.org/10.1016/0022-2836(87)90697-8" @default.
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