Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078722467> ?p ?o ?g. }
- W2078722467 endingPage "2367" @default.
- W2078722467 startingPage "2359" @default.
- W2078722467 abstract "Duct cells of the pancreas are thought to include latent progenitors of islet endocrine cells that can be induced to differentiate by appropriate morphogens. Here we developed a method for isolating pancreatic ductal epithelial cells from adult mice that overcomes the shortcomings of previous methods. Pancreatic ductal cells were grown in serum-free DMEM/F12 medium in the presence of cholera toxin or 8-bromo-cyclic adenosine monophosphate, which is known to be an intracellular cAMP generator. Single cell cloning was performed by limiting dilution in serum-free medium. The isolated clonal cells expressed high levels of cytokeratin and Ipf1 (formerly known as Pdx-1). Adenovirus-mediated expression of ngn3 (also known as Neurog3) and Ptf1a in these cells induced expression of insulin and somatostatin, and of carboxypeptidase A, respectively. Furthermore, albumin production was induced by dexamethasone or by long-term culture in serum-containing medium. Stimulation of the cAMP-dependent signalling allowed us to isolate clonal pancreatic ductal cells from adult mice. These cells are able to partially differentiate into endocrine cells, exocrine cells and hepatocyte-like cells and are therefore considered to have the characteristics of endodermal progenitor cells." @default.
- W2078722467 created "2016-06-24" @default.
- W2078722467 creator A5001334483 @default.
- W2078722467 creator A5003175073 @default.
- W2078722467 creator A5014959654 @default.
- W2078722467 creator A5039294674 @default.
- W2078722467 creator A5045237065 @default.
- W2078722467 creator A5055988870 @default.
- W2078722467 creator A5056801689 @default.
- W2078722467 creator A5061418381 @default.
- W2078722467 creator A5078107899 @default.
- W2078722467 date "2006-08-08" @default.
- W2078722467 modified "2023-10-18" @default.
- W2078722467 title "Stimulation of cAMP signalling allows isolation of clonal pancreatic precursor cells from adult mouse pancreas" @default.
- W2078722467 cites W1518831632 @default.
- W2078722467 cites W1559720000 @default.
- W2078722467 cites W1587712169 @default.
- W2078722467 cites W1958779837 @default.
- W2078722467 cites W1967705577 @default.
- W2078722467 cites W1974828844 @default.
- W2078722467 cites W1982781304 @default.
- W2078722467 cites W1999179141 @default.
- W2078722467 cites W2003085149 @default.
- W2078722467 cites W2005688198 @default.
- W2078722467 cites W2014145783 @default.
- W2078722467 cites W2015938531 @default.
- W2078722467 cites W2019032880 @default.
- W2078722467 cites W2020687437 @default.
- W2078722467 cites W2028188226 @default.
- W2078722467 cites W2029167057 @default.
- W2078722467 cites W2049318896 @default.
- W2078722467 cites W2067389522 @default.
- W2078722467 cites W2072923149 @default.
- W2078722467 cites W2077633291 @default.
- W2078722467 cites W2084488354 @default.
- W2078722467 cites W2089584400 @default.
- W2078722467 cites W2124307629 @default.
- W2078722467 cites W2142182692 @default.
- W2078722467 cites W2146383332 @default.
- W2078722467 cites W2154650642 @default.
- W2078722467 cites W2163479796 @default.
- W2078722467 cites W2163592665 @default.
- W2078722467 cites W2164940024 @default.
- W2078722467 cites W2172051454 @default.
- W2078722467 cites W2178690468 @default.
- W2078722467 cites W2461361678 @default.
- W2078722467 doi "https://doi.org/10.1007/s00125-006-0372-7" @default.
- W2078722467 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16896938" @default.
- W2078722467 hasPublicationYear "2006" @default.
- W2078722467 type Work @default.
- W2078722467 sameAs 2078722467 @default.
- W2078722467 citedByCount "28" @default.
- W2078722467 countsByYear W20787224672012 @default.
- W2078722467 countsByYear W20787224672013 @default.
- W2078722467 countsByYear W20787224672014 @default.
- W2078722467 countsByYear W20787224672016 @default.
- W2078722467 countsByYear W20787224672017 @default.
- W2078722467 countsByYear W20787224672018 @default.
- W2078722467 countsByYear W20787224672019 @default.
- W2078722467 countsByYear W20787224672022 @default.
- W2078722467 crossrefType "journal-article" @default.
- W2078722467 hasAuthorship W2078722467A5001334483 @default.
- W2078722467 hasAuthorship W2078722467A5003175073 @default.
- W2078722467 hasAuthorship W2078722467A5014959654 @default.
- W2078722467 hasAuthorship W2078722467A5039294674 @default.
- W2078722467 hasAuthorship W2078722467A5045237065 @default.
- W2078722467 hasAuthorship W2078722467A5055988870 @default.
- W2078722467 hasAuthorship W2078722467A5056801689 @default.
- W2078722467 hasAuthorship W2078722467A5061418381 @default.
- W2078722467 hasAuthorship W2078722467A5078107899 @default.
- W2078722467 hasBestOaLocation W20787224671 @default.
- W2078722467 hasConcept C103395026 @default.
- W2078722467 hasConcept C126322002 @default.
- W2078722467 hasConcept C134018914 @default.
- W2078722467 hasConcept C201750760 @default.
- W2078722467 hasConcept C2778148616 @default.
- W2078722467 hasConcept C2778764654 @default.
- W2078722467 hasConcept C28328180 @default.
- W2078722467 hasConcept C46699223 @default.
- W2078722467 hasConcept C71315377 @default.
- W2078722467 hasConcept C71924100 @default.
- W2078722467 hasConcept C86803240 @default.
- W2078722467 hasConcept C95444343 @default.
- W2078722467 hasConceptScore W2078722467C103395026 @default.
- W2078722467 hasConceptScore W2078722467C126322002 @default.
- W2078722467 hasConceptScore W2078722467C134018914 @default.
- W2078722467 hasConceptScore W2078722467C201750760 @default.
- W2078722467 hasConceptScore W2078722467C2778148616 @default.
- W2078722467 hasConceptScore W2078722467C2778764654 @default.
- W2078722467 hasConceptScore W2078722467C28328180 @default.
- W2078722467 hasConceptScore W2078722467C46699223 @default.
- W2078722467 hasConceptScore W2078722467C71315377 @default.
- W2078722467 hasConceptScore W2078722467C71924100 @default.
- W2078722467 hasConceptScore W2078722467C86803240 @default.
- W2078722467 hasConceptScore W2078722467C95444343 @default.
- W2078722467 hasIssue "10" @default.
- W2078722467 hasLocation W20787224671 @default.
- W2078722467 hasLocation W20787224672 @default.