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- W2078728612 abstract "OBJECTIVE: To determine if outbred mice are more sensitive to in vitro stress as measured by the one cell mouse embryo assay (MEA). DESIGN: Laboratory experiment comparing response of 4 mouse strains to 3 adverse culture conditions. MATERIALS AND METHODS: Fresh one-cell embryos from outbred (CF1), inbred (FVB), B6/CBA hybrid (F1) and frozen hybrid embryos from Embryo Tech™ (F2) were compared in a standard MEA assay using six doses of each of the 3 different in vitro stressors with studies in triplicate. Embryos were cultured in microdrops of Global media and exposed to 6 doses ranging from: (A) 0 to 10 μM cumene peroxide in mineral oil, (B) 0 to 0.003% TX-100 in culture media, and (C) 275 to 450 mOsm culture media. Percentage of expanded blastocysts (EB) at 96 hours and LC50 values (dose at which EB was 50 % of the control EB for that strain) were determined. Data were analyzed with ANOVA and reported as mean ± SD. RESULTS: CF1 embryos were able to detect less than half of the cumene peroxidase and TX-100 compared to all other strains tested. No difference in strains was noted for changes in osmolarity.Tabled 1LC 50 values (mean) for mouse strainsF1 (LC 50)FVB (LC 50)F2 (LC 50)CF1 (LC 50)p-ValueCumene Peroxide (μM)7.0 ± 1.76.8 ± 1.26.6 ± 2.42.3 ± 0.70.022∗p<0.05.TX-100 (% × 10-3)1.2 ± 0.061.8 ± 0.21.8 ± 0.040.7 ± 0.050.0010∗p<0.05.Osmolarity (mOsm)332 ± 6328 ± 7325 ± 8323 ± 8NS∗ p<0.05. Open table in a new tab CONCLUSION: CF1 embryos are genetically complex and diverse (like human embryos) and are more sensitive to toxins than either inbred or hybrid mouse embryos. Further research is warranted to determine if the current practice of using F2 hybrid embryos is optimal for research and quality control of media and supplies for human IVF." @default.
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- W2078728612 date "2010-09-01" @default.
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- W2078728612 title "Mouse embryos and in vitro stress: does mouse strain matter?" @default.
- W2078728612 doi "https://doi.org/10.1016/j.fertnstert.2010.07.224" @default.
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