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- W2078750152 abstract "The p38 mitogen activated protein kinase (MAPK) has emerged as a target for treating inflammatory diseases, like rheumatoid arthritis (RA). Expression of p38δ is induced in rheumatoid arthritis synovial fibroblasts (RASFs) by a cytokine-independent pathway substantially different from other MAPK pathways. To identify inhibitors of p38δ MAPK, we developed a direct ELISA assay based on a previously described p38α assay for monitoring the phosphorylation of ATF-2. This work presents a straightforward assay for evaluating the potency of small-molecule inhibitors. To validate the assay under optimized conditions, we used reference compounds and achieved results comparable to published data." @default.
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- W2078750152 date "2012-07-01" @default.
- W2078750152 modified "2023-10-17" @default.
- W2078750152 title "Development of a p38δ mitogen activated protein kinase ELISA assay for the quantitative determination of inhibitor activity" @default.
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- W2078750152 doi "https://doi.org/10.1016/j.jpba.2012.03.008" @default.
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